Gene: [01p32/RLF] rearranged L-myc fusion protein;


COM

 The sequence, included in this entry, corresponds only partly to the gene/protein in question since so far complete cDNA of the gene has not been yet published."

SUM

 This protein is related to members of the MYC family. Initially, it was revealed as a part of chimeric polypeptides with amino-terminal sequences derived from a novel gene, named RLF (Rearranged Lmyc Fusion) since its protein has been found to be linked with the Lmyc protein only in cells with an amplified LMYC gene (Makela-1991). Also, since the chimeric products of the RLF/MYCL fusion gene were found in different small cell lung carcinoma (SCLC) cell lines, the authors suggested a role for the fusion protein in oncogenesis. The authors cloned a cDNA encoding the MYCLRF protein itself, and also established a physical linkage between this gene and L-myc-1 on Chr 1 within the band p32. The both genes appeared to be separated by less than 800 kb."

COD

 "<* TITLE:> [PROT= 96? aa (MW= ?) from cDNA= 300 bp]
<* REFER_1:> [Makela TP &:1991]
/IDP: Makela TP, Saksela K, Evan G, Alitalo K;
'A fusion protein formed by L-myc and a novel gene in SCLC';
EMBO J, 10, N6, 1331-1335, 1991\
/IDS_1: emb:X58480^HSRLF| cDNA= 300 bp;
'Human rlf mRNA for l-myc associated fusion protein';
//note: seq_from cell_line GLC28\
/Date: May-15-1991\
/Submitted_by: Maekelae TP (Cancer Biology Lab, Depts Virology and
Pathology, University of Helsinki, Haartmaninkatu 3, 00290
Helsinki, Finland);
//date: Mar-22-1991; //note: only 1..249 nt\
/Duplicate: cbi:36069-070\
/Note_1: The last name 'Maekelae' indicated in this emb:entry should
probably be 'Makela' as in the original publication.\
/Note_2: The following comment is selected from this emb:entry -
'A breakpoint is found at position 249 bp, and corresponds to nu-
cleotide 796 L-myc of Kaye F, Battey J, Nau M, Brooks B, Seifter E,
De Greve J, Birrer M, Sausville E and Minna J. Mol Cell Biol, 8,
186-195 (1988).'
However, without additional clarifications, this comment may orig-
inates a confusion (see '/Note:' in 'Sequence' below).\
%-----------------------------------------------------------------------
<* LANDMARKS>
{seg(<1..12) == 5'-UTL(=12) //: 5'-UnTransLated region (possibly
incomplete);
seg(13..300>) == CDS(=288) //: CoDing_Segment (for 96 aa only);
pos:13 == TLI_1 //: TransLation START;
pos:246|247 == BFP_1 //: Break-Fusion_Point}
%-----------------------------------------------------------------------
<* SEQUENCE:> [cDNA= 300 bp]
/Note: The most probable site of the mentioned 'break-fusion_point' is
restricted by nucleotides #246|247, in the ref1 sequence, and by
#792|793, in the ref2 sequence, where ref2 is (Kaye F &:1988).
Also, in the ref2 sequence, seg(784..795) is a terminal segment of
the intron A (or 1) while seg(796..804) is a 5'-untranslated part
of the exon 2 (see 'Gene_structure' in GEM:01p32/MYCL1).
Thus, the L-myc-1's part begins at the position #83 in the fusion
protein, or at the position #259 in the nucleotide sequence below.\
%-------
ids1: 1=cgccgtggga ag=12_
%-------
pep1: 1= M A D G K G D A A A V A G A G =
ids1: 13=atg gca gac gga aag gga gac gcc gcc gct gtc gcc ggg gct ggg=
pep1: 16= A E A P A V A G A G D G V E T =
ids1: 58=gct gag gct ccg gcg gta gcg gga gcc gga gat gga gtc gag act=
pep1: 31= E S M V R G H R P V S P A P G =
ids1:103=gag tcc atg gtt cgg ggt cat cgc ccc gta tct cca gcg ccg gga=
pep1: 46= A S G L R P C L W Q L E T E L =
ids1:148=gcc tcg gga ctg cgg ccg tgt ctg tgg cag ctg gag aca gag ctg=
pep1: 61= R E Q E V S E V S S L N Y C R =
ids1:193=agg gag caa gag gtg tcg gag gtc tca tct ttg aac tac tgc cgg=
pep1: 76= S F C =78_
ids1:238=AGc Ttc TGC=246_
#ref2:784=TCc Ctc CCG=792_
%-------
pep1: 79= Q G A D M D Y D S Y Q H Y F Y =
ids1:247=cag gga gcg gac atg gac tac gac tcg tac cag cac tat ttc tac=
#ref2:793=cag gga gcg gac atg gac tac gac tcg tac cag cac tat ttc tac=
#pep2: 1=_________________M D Y D S Y Q H Y F Y =
//note: L-myc-1 protein ^ is as follows ..\
%-------
pep1: 94= D Y D =96\
ids1:292=gac tat gac=300\
#pep2: 12= D Y D C =15..|
#ref2:838=gac tat gac tgc=849..|"

PSG

 GEM:00.0/RLFP.

REL

 GEM:01p32/MYCL1.

REF

 COM,SQN "Kaye F &: Mol Cell Biol, 8, 186-195, 1988
CLO,COD,SEQ,LOC,MOL "Makela TP &: Mol Cell Biol, 11, 4015-4021, 1991

KEY

 onc, fus

CLA

 coding, basic

LOC

 01 p32

MIM

 MIM: 180610