HGNC Approved Gene Symbol: AOAH
Cytogenetic location: 7p14.2 Genomic coordinates (GRCh38): 7:36,512,941-36,724,494 (from NCBI)
Acyloxyacyl hydrolase (AOAH) is a 2-subunit lipase present in phagocytic cells. The enzyme specifically hydrolyzes the secondary acyl chains of the lipopolysaccharide found in the walls of gram-negative bacteria (summary by Whitmore et al., 1994).
Hagen et al. (1991) cloned and characterized cDNA for human AOAH and showed that its 2 subunits are translated from a single mRNA molecule about 2.2 kb long.
Whitmore et al. (1994) stated the action of acyloxyacyl hydrolase on lipopolysaccharide (or endotoxin) suggests that it modulates the host's inflammatory response to gram-negative bacteria. This hypothesis was supported by studies showing that the deacylation of lipopolysaccharide by AOAH in vitro greatly reduces its toxicity and activity.
By fluorescence in situ hybridization, Whitmore et al. (1994) mapped the AOAH gene to 7p14-p12.
Hagen, F. S., Grant, F. J., Kuijper, J. L., Slaughter, C. A., Moomaw, C. R., Orth, K., O'Hara, P. J., Munford, R. S. Expression and characterization of recombinant human acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides. Biochemistry 30: 8415-8423, 1991. [PubMed: 1883828] [Full Text: https://doi.org/10.1021/bi00098a020]
Whitmore, T. E., Mathewes, S. L., O'Hara, P. J., Durnam, D. M. Chromosomal localization of the acyloxyacyl hydrolase (AOAH) gene to 7p14-p12 using fluorescence in situ hybridization. Genomics 21: 457-458, 1994. [PubMed: 8088847] [Full Text: https://doi.org/10.1006/geno.1994.1298]