Alternative titles; symbols
HGNC Approved Gene Symbol: CPB1
Cytogenetic location: 3q24 Genomic coordinates (GRCh38): 3:148,827,811-148,860,187 (from NCBI)
Yamamoto et al. (1992) isolated a human cDNA for CPB1, which they called PCPB. The deduced 416-amino acid preproenzyme (EC 3.4.17.2) has a 15-amino acid signal peptide and a 95-amino acid activation peptide. It shares 76% and 84% sequence identity with the rat and bovine homologs, respectively. DNA blot analysis indicated that the PCPB mRNA is transcribed from a single locus. Northern blot analysis demonstrated pancreas-specific expression.
By use of SDS-gel electrophoresis and Western blot analysis, Fernstad et al. (1993) identified several different PCPB isoforms.
Yamamoto et al. (1992) noted that pancreatic carboxypeptidase B was originally described and characterized as pancreas-specific protein (PSAP), a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. The protein constitutes about 2% of total pancreatic cytosolic proteins (Pousette et al., 1988).
Gross (2014) mapped the CPB1 gene to chromosome 3q24 based on an alignment of the CPB1 sequence (GenBank BC015338) with the genomic sequence (GRCh37).
Fernstad, R., Kylander, C., Tsai, L., Tyden, G., Pousette, A. Isoforms of procarboxypeptidase B, (pancreas-specific protein, PASP) in human serum, pancreatic tissue and juice. Scand. J. Clin. Lab. Invest. 53 (suppl. 213): 9-17, 1993.
Gross, M. B. Personal Communication. Baltimore, Md. 6/24/2014.
Pousette, A., Fernstad, R., Skoldefors, H., Carlstrom, K. Novel assay for pancreatic cellular damage: 1. Characterization of protein profiles in human pancreatic cytosol and purification and characterization of a pancreatic specific protein. Pancreas 3: 421-426, 1988. [PubMed: 2459692]
Yamamoto, K. K., Pousette, A., Chow, P., Wilson, H., El Shami, S., French, C. K. Isolation of a cDNA encoding a human serum marker for acute pancreatitis: identification of pancreas-specific protein as pancreatic procarboxypeptidase B. J. Biol. Chem. 267: 2575-2581, 1992. [PubMed: 1370825]