Alternative titles; symbols
HGNC Approved Gene Symbol: CDC27
Cytogenetic location: 17q21.32 Genomic coordinates (GRCh38): 17:47,117,703-47,189,295 (from NCBI)
CDC27 is 1 of several subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle (summary by Jorgensen et al., 2001).
Tugendreich et al. (1993) described a strategy for quickly identifying and positionally mapping human homologs of yeast genes in order to cross-reference the rich biologic and genetic information concerning yeast genes to mammalian species. Optimized computer search methods were developed to scan the rapidly expanding expressed sequence tag (EST) database of Venter and colleagues (Adams et al., 1992) to find human open reading frames related to yeast protein sequences. The corresponding human cDNA was then used to obtain a high-resolution map position on human and mouse chromosomes, providing the links between yeast genetic analysis and mapped mammalian loci. In this way, Tugendreich et al. (1993) identified a human homolog of CDC27 of Saccharomyces cerevisiae. Human CDC27 encodes an 823-amino acid protein with global similarity to its fungal homologs.
Using a library of endoribonuclease-prepared short interfering RNAs (esiRNAs), Kittler et al. (2004) identified 37 genes required for cell division, one of which was CDC27. These 37 genes included several splicing factors for which knockdown generates mitotic spindle defects. In addition, a putative nuclear-export terminator was found to speed up cell proliferation and mitotic progression after knockdown.
Tugendreich et al. (1993) mapped the CDC27 gene to human chromosome 17 and mouse chromosome 11 between the PKCA gene (176960) at 17q22-q23.2 and the ERBB2 gene (164870) at 17q12-q21. The assignment to human chromosome 17 was achieved by PCR analysis of a panel of somatic cell hybrids; the mapping to chromosome 11 between the murine homologs of the PKCA and ERBB2 genes was done by linkage analysis. That CDC27 is located between these genes in the human was inferred from the strong homology of synteny.
Adams, M. D., Dubnick, M., Kerlavage, A. R., Moreno, R., Kelley, J. M., Utterback, T. R., Nagle, J. W., Fields, C., Venter, J. C. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. [PubMed: 1538749] [Full Text: https://doi.org/10.1038/355632a0]
Jorgensen, P. M., Graslund, S., Betz, R., Stahl, S., Larsson, C., Hoog, C. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene 262: 51-59, 2001. [PubMed: 11179667] [Full Text: https://doi.org/10.1016/s0378-1119(00)00511-4]
Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Korn, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004. [PubMed: 15616564] [Full Text: https://doi.org/10.1038/nature03159]
Tugendreich, S., Boguski, M. S., Seldin, M. S., Hieter, P. Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base. Proc. Nat. Acad. Sci. 90: 10031-10035, 1993. [PubMed: 8234252] [Full Text: https://doi.org/10.1073/pnas.90.21.10031]