Alternative titles; symbols
Ming et al. (1976) demonstrated that a factor essential to the normal mammalian cell cycle is located on human chromosome 3. AF8 Syrian hamster cells have a temperature-sensitive mutation; they grow normally at 33.5 degrees F, but at 39 degrees are blocked in mid-G1. When these cells are fused with Lesch-Nyhan (see 300322) fibroblasts transformed by simian virus 40, the hybrid cells grow at 39 degrees. Ming et al. (1976) observed preferential retention of human chromosome 3 in all hybrid clones that would grow at 39 degrees and often only that chromosome was retained. This indicates that a factor (or factors) concerned with the mammalian cell cycle at the G1 stage is carried by chromosome 3. Other temperature sensitivity complementation loci, all cell cycle specific, are located on chromosomes 9 (187290), 14 (187310), 4 (187320), and 6 (187330). See 313650 for description of an X-linked temperature-sensitive mutation of mouse and hamster (complemented by the human X-chromosome). Ashihara et al. (1978) showed that the tsAF8 cells are blocked, at the nonpermissive temperature, at a specific point in the mid-G1 phase of the cell cycle. Rossini and Baserga (1978) showed that the temperature-sensitive defect in AF8 cells is associated with loss of RNA polymerase II activity. One subunit of RNA polymerase II (180660) is coded by chromosome 17p.
Ashihara, T., Chang, S. D., Baserga, R. Constancy of the shift-up point in two temperature-sensitive mammalian cell lines that assert in G(1). J. Cell. Physiol. 96: 15-21, 1978. [PubMed: 659518] [Full Text: https://doi.org/10.1002/jcp.1040960103]
Ming, P.-M. L., Chang, H. L., Baserga, R. Release by human chromosome 3 of the block at G1 of the cell cycle, in hybrids between tsAF8 hamster and human cells. Proc. Nat. Acad. Sci. 73: 2052-2055, 1976. [PubMed: 180537] [Full Text: https://doi.org/10.1073/pnas.73.6.2052]
Rossini, M., Baserga, R. RNA synthesis in cell cycle-specific temperature sensitive mutant from hamster cell line. Biochemistry 17: 858-863, 1978. [PubMed: 629937] [Full Text: https://doi.org/10.1021/bi00598a017]
Simchen, G. Cell cycle mutants. Annu. Rev. Genet. 12: 161-191, 1978. [PubMed: 106766] [Full Text: https://doi.org/10.1146/annurev.ge.12.120178.001113]