Alternative titles; symbols
HGNC Approved Gene Symbol: CDKN3
Cytogenetic location: 14q22.2 Genomic coordinates (GRCh38): 14:54,397,007-54,420,218 (from NCBI)
Gyuris et al. (1993) used the interaction trap, a yeast genetic selection for interacting proteins, to isolate a gene they called human cyclin-dependent kinase interactor-1 (CDI1). In yeast, Cdi1 interacts with cyclin-dependent kinases, including human CDC2 (116940), CDK2 (116953), and CDK3 (123828), but not with CDK4 (123829). In HeLa cells, CDI1 is expressed at the G1 to S transition, and the protein forms stable complexes with CDK2. In vitro, CDI1 removes phosphate from tyrosine residues in model substrates, but a mutant protein that bears a lesion in the putative active site cysteine does not. Overexpression of wildtype CDI1 delays progression through the cell cycle in yeast and HeLa cells; delay is dependent on CDI1 phosphatase activity.
Hannon et al. (1994) isolated the CDKN3 gene, which they referred to as kinase-associated phosphatase (KAP), by virtue of its association with CDK2 (116953). This protein is a dual-specificity protein phosphatase that inhibits cell cycle progression, although its exact mechanism of action was unknown (Gyuris et al., 1993).
Demetrick et al. (1995) mapped the CDKN3 gene to chromosome 14q22 by fluorescence in situ hybridization.
Demetrick, D. J., Matsumoto, S., Hannon, G. J., Okamoto, K., Xiong, Y., Zhang, H., Beach, D. H. Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3). Cytogenet. Cell Genet. 69: 190-192, 1995. [PubMed: 7698009] [Full Text: https://doi.org/10.1159/000133960]
Gyuris, J., Golemis, E., Chertkov, H., Brent, R. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell 75: 791-803, 1993. [PubMed: 8242750] [Full Text: https://doi.org/10.1016/0092-8674(93)90498-f]
Hannon, G. J., Casso, D., Beach, D. KAP: a dual specificity phosphatase that interacts with cyclin-dependent kinases. Proc. Nat. Acad. Sci. 91: 1731-1735, 1994. [PubMed: 8127873] [Full Text: https://doi.org/10.1073/pnas.91.5.1731]