Alternative titles; symbols
HGNC Approved Gene Symbol: CRIP1
Cytogenetic location: 14q32.33 Genomic coordinates (GRCh38): 14:105,486,886-105,488,947 (from NCBI)
Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine- and glycine-rich protein-1 (CSRP1; 123876), rhombotin-1 (RBTN1; 186921), rhombotin-2 (RBTN2; 180385), and rhombotin-3 (RBTN3; 180386). CRIP may be involved in intestinal zinc transport (Hempe and Cousins, 1991).
Tsui et al. (1994) isolated a human adult heart cDNA whose open reading frame has 89% sequence similarity with the open reading frames of rat and mouse Crip. The deduced protein, which the authors called cysteine-rich heart protein (CRHP), is 97% identical to mouse Crip. The 77-amino acid CRHP contains a single LIM motif and a glycine-rich domain. Northern blot analysis detected an approximately 670-bp CRHP mRNA in human fetal heart. Rat Crip was highly expressed in small intestine, with lower levels in adult heart and spleen; the authors found that rat Crip is developmentally regulated in heart.
By RT-PCR using human small intestine RNA and oligonucleotides based on the CRHP cDNA isolated by Tsui et al. (1994), Khoo et al. (1997) cloned a cDNA encoding CRIP. Southern blot analysis suggested that there are 3 copies of the CRIP gene in the human genome. RT-PCR detected CRIP mRNA in human monocytes. Purified, bacterially expressed CRIP protein has a molecular mass of 8,390 Da by mass spectrometry.
Garcia-Barcelo et al. (1998) mapped the human CRIP1 gene to 7q11.23 by analysis of somatic cell hybrids and radiation hybrids. However, by sequence analysis, (Zhou et al., 2002) placed Crip close to Crp2 (601183) and Mta-1 (603526) approximately 70 kb downstream of the Igh locus on mouse chromosome 12 in a region of syntenic homology with human 14q32.33. In addition, in the mouse and rat, Birkenmeier and Gordon (1986) had found that the gene encoding Crip is closely linked to the Igh constant region on chromosome 12.
Birkenmeier, E. H., Gordon, J. I. Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus. Proc. Nat. Acad. Sci. 83: 2516-2520, 1986. [PubMed: 3085096] [Full Text: https://doi.org/10.1073/pnas.83.8.2516]
Garcia-Barcelo, M., Tsui, S. K. W., Chim, S. S., Fung, K. P., Lee, C. Y., Waye, M. M. Y. Mapping of the human cysteine-rich intestinal protein gene CRIP1 to the human chromosomal segment 7q11.23. Genomics 47: 419-422, 1998. [PubMed: 9480758] [Full Text: https://doi.org/10.1006/geno.1997.5134]
Hempe, J. M., Cousins, R. J. Cysteine-rich intestinal protein binds zinc during transmucosal zinc transport. Proc. Nat. Acad. Sci. 88: 9671-9674, 1991. [PubMed: 1946385] [Full Text: https://doi.org/10.1073/pnas.88.21.9671]
Khoo, C., Blanchard, R. K., Sullivan, V. K., Cousins, R. J. Human cysteine-rich intestinal protein: cDNA cloning and expression of recombinant protein and identification in human peripheral blood mononuclear cells. Protein Expr. Purif. 9: 379-387, 1997. [PubMed: 9126610] [Full Text: https://doi.org/10.1006/prep.1996.0709]
Tsui, S. K. W., Yam, N. Y. H., Lee, C. Y., Waye, M. M. Y. Isolation and characterization of a cDNA that codes for a LIM-containing protein which is developmentally regulated in heart. Biochem. Biophys. Res. Commun. 205: 497-505, 1994. [PubMed: 7999070] [Full Text: https://doi.org/10.1006/bbrc.1994.2693]
Zhou, J., Ashouian, N., Delepine, M., Matsuda, F., Chevillard, C., Riblet, R., Schildkraut, C. L., Birshtein, B. K. The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression. Proc. Nat. Acad. Sci. 99: 13693-13698, 2002. [PubMed: 12370427] [Full Text: https://doi.org/10.1073/pnas.212392399]