Alternative titles; symbols
HGNC Approved Gene Symbol: CSRP1
Cytogenetic location: 1q32.1 Genomic coordinates (GRCh38): 1:201,483,530-201,507,123 (from NCBI)
The human gene encoding cysteine-rich protein (CSRP) is a highly conserved, cell cycle-regulated gene that is induced in the immediate early response to serum repletion in serum-starved, noncycling cells. The LIM/double zinc finger motif found in cysteine-rich protein is found in an expanding group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation (summary by Wang et al., 1992).
By screening a human placenta cDNA library with a human prolactin (PRL; 176760) cDNA, Liebhaber et al. (1990) isolated cDNAs encoding CRP. The deduced 193-amino acid protein has 2 repeats of a domain that consists of 2 putative zinc fingers immediately followed by a glycine-rich motif containing a high proportion of aromatic and basic residues. CRP does not share regions of significant structural similarity with PRL. Northern blot and RT-PCR analyses detected CRP expression in all human nucleated tissues and cell lines examined; CRP is expressed as a 1.8-kb transcript.
Wang et al. (1992) cloned the human CRP genomic sequence. They showed that CRP is a primary response gene in both human fibroblasts and mouse Balb/c 3T3 cells; in the mouse cells, the kinetic profile of its induction closely paralleled that of c-myc (190080).
Wang et al. (1992) determined that the CRP gene contains 6 exons, with a 10.4-kb first intron, and spans approximately 23.2 kb from the cap site to the polyadenylation site.
Other members of the LIM/double zinc finger group include cysteine-rich intestinal protein (CRIP; 123875), CSRP2 (601871), CSRP3 (600824), and the rhombotin genes RBTN1 (186921), RBTN2 (180385), and RBTN3 (180386). Weiskirchen et al. (1995) described the CRP family of LIM domain proteins.
Using a panel of human/rodent somatic cell hybrids, Wang et al. (1992) assigned the CSRP gene to chromosome 1. By in situ hybridization of (3)H-labeled cDNA, they regionalized the gene to 1q24-q32. A common MspI polymorphism was found and mapped to intron 4 of the CSRP gene. By FISH, Erdel and Weiskirchen (1998) mapped the CSRP1 to 1q32.
Alli and Consalez (1998) mapped the Csrp gene to mouse chromosome 3 by haplotype and linkage analysis of progeny from an interspecific backcross panel.
Alli, C., Consalez, G. G. Linkage mapping of Csrp to proximal mouse chromosome 3. Mammalian Genome 9: 172 only, 1998. [PubMed: 9457685] [Full Text: https://doi.org/10.1007/s003359900714]
Erdel, M., Weiskirchen, R. Assignment of CSRP1 encoding the LIM domain protein CRP1, to human chromosome 1q32 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 83: 10-11, 1998. [PubMed: 9925910] [Full Text: https://doi.org/10.1159/000015152]
Liebhaber, S. A., Emery, J. G., Urbanek, M., Wang, X., Cooke, N. E. Characterization of a human cDNA encoding a widely expressed and highly conserved cysteine-rich protein with an unusual zinc-finger motif. Nucleic Acids Res. 18: 3871-3879, 1990. [PubMed: 2115670] [Full Text: https://doi.org/10.1093/nar/18.13.3871]
Wang, X., Lee, G., Liebhaber, S. A., Cooke, N. E. Human cysteine-rich protein: a member of the LIM/double-finger family displaying coordinate serum induction with c-myc. J. Biol. Chem. 267: 9176-9184, 1992. [PubMed: 1374386]
Wang, X., Ray, K., Szpirer, J., Levan, G., Liebhaber, S. A., Cooke, N. E. Analysis of the human cysteine-rich protein gene (CSRP), assignment to chromosome 1q24-1q32, and identification of an associated MspI polymorphism. Genomics 14: 391-397, 1992. [PubMed: 1385304] [Full Text: https://doi.org/10.1016/s0888-7543(05)80231-9]
Weiskirchen, R., Pino, J. D., Macalma, T., Bister, K., Beckerle, M. C. The cysteine-rich protein family of highly related LIM domain proteins. J. Biol. Chem. 270: 28946-28954, 1995. [PubMed: 7499425] [Full Text: https://doi.org/10.1074/jbc.270.48.28946]