Alternative titles; symbols
HGNC Approved Gene Symbol: HSPA7
Cytogenetic location: 1q23.3 Genomic coordinates (GRCh38): 1:161,606,172-161,608,550 (from NCBI)
HSPA7 is transcribed in response to heat shock, but it does not encode a full-length functional protein (Parsian et al., 2000).
Using the coding region of Drosophila Hsp70 to screen a human genomic phage library, Voellmy et al. (1985) cloned a segment of a gene encoding a 70-kD heat shock protein, designated HSP70B by Schiller et al. (1988).
Leung et al. (1990) found high sequence homology between HSP70B (HSPA7) and HSPA6 (140555), formerly designated HSP70B-prime. Leung et al. (1992) stated that both HSPA6 and HSPA7 represent functional genes, as determined by analyses of mRNA from heat-shocked human cells using sequence-specific oligonucleotides. After heat shock at 45 degrees C, HSPA6 mRNA was detected in fibroblast, HeLa, and Daudi cells, whereas HSPA7 mRNA was detected only in fibroblasts.
Parsian et al. (2000) presented evidence that HSPA7 does not encode a full-length functional protein. They did not detect orthologs of HSPA7 or HSPA6 in mouse by Southern blot analysis.
Using Xenopus and mammalian cells, Voellmy et al. (1985) demonstrated that the expression of HSPA7 mRNA is heat regulated. Using RT-PCR, Parsian et al. (2000) detected the expression of HSPA7 and HSPA6 only after heat shock in human W138 and HeLa cells. HSPA6 was more strongly expressed following heat shock.
Voellmy et al. (1985) determined that the promoter region of the HSPA7 gene is GC-rich and lacks CCAAT and TATA elements. Schiller et al. (1988) extended the upstream nucleotide sequence of HSPA7 and identified 4 potential heat shock transcription factor recognition sites. They also identified upstream CCAAT-like sequence and many potential SP1 (189906)-binding sites.
By hybridization analyses of a somatic cell hybrid DNA panel, Leung et al. (1992) found that HSPA6 and HSPA7 localize to 1q. A BamHI polymorphism in the HSPA7 gene was present in a predominantly Asian population.
Grosz et al. (1992) concluded that bovine HSP70-4 is homologous to HSPA6 or HSPA7 because it is syntenic with amylase-1 (104700) and PGM1 (171900), both of which are on human chromosome 1.
Using FISH, Parsian et al. (2000) mapped the HSPA6 and HSPA7 genes to within 5 to 10 Mb of each other on chromosome 1q23.1.
Brzustowicz et al. (2002) stated that the HSPA6 and HSPA7 genes are located on chromosome 1q22, according to sequence data provided by the Human Genome Project, and are in close proximity to a susceptibility locus for schizophrenia (604906).
Brzustowicz, L. M., Hayter, J. E., Hodgkinson, K. A., Chow, E. W. C., Bassett, A. S. Fine mapping of the schizophrenia susceptibility locus on chromosome 1q22. Hum. Hered. 54: 199-209, 2002. [PubMed: 12771552] [Full Text: https://doi.org/10.1159/000070665]
Grosz, M. D., Womack, J. E., Skow, L. C. Syntenic conservation of HSP70 genes in cattle and humans. Genomics 14: 863-868, 1992. [PubMed: 1478667] [Full Text: https://doi.org/10.1016/s0888-7543(05)80106-5]
Leung, T. K. C., Hall, C., Rajendran, M., Spurr, N. K., Lim, L. The human heat-shock genes HSPA6 and HSPA7 are both expressed and localize to chromosome 1. Genomics 12: 74-79, 1992. [PubMed: 1346391] [Full Text: https://doi.org/10.1016/0888-7543(92)90409-l]
Leung, T. K. C., Rajendran, M. Y., Monfries, C., Hall, C., Lim, L. The human heat-shock protein family: expression of a novel heat-inducible HSP70 (HSP70B-prime) and isolation of its cDNA and genomic DNA. Biochem. J. 267: 125-132, 1990. [PubMed: 2327978] [Full Text: https://doi.org/10.1042/bj2670125]
Parsian, A. J., Sheren, J. E., Tao, T. Y., Goswami, P. C., Malyapa, R., Van Rheeden, R., Watson, M. S., Hunt, C. R. The human Hsp70B gene at the HSPA7 locus of chromosome 1 is transcribed but non-functional. Biochim. Biophys. Acta 1494: 201-205, 2000. [PubMed: 11072087] [Full Text: https://doi.org/10.1016/s0167-4781(00)00203-7]
Schiller, P., Amin, J., Ananthan, J., Brown, M. E., Scott, W. A., Voellmy, R. Cis-acting elements involved in the regulated expression of a human HSP70 gene. J. Molec. Biol. 203: 97-105, 1988. [PubMed: 3184191] [Full Text: https://doi.org/10.1016/0022-2836(88)90094-0]
Voellmy, R., Ahmed, A., Schiller, P., Bromley, P., Rungger, D. Isolation and functional analysis of a human 70,000-dalton heat shock protein gene segment. Proc. Nat. Acad. Sci. 82: 4949-4953, 1985. [PubMed: 3927293] [Full Text: https://doi.org/10.1073/pnas.82.15.4949]