* 146735

INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 6; IGFBP6


Alternative titles; symbols

IBP6


HGNC Approved Gene Symbol: IGFBP6

Cytogenetic location: 12q13.13     Genomic coordinates (GRCh38): 12:53,097,667-53,102,340 (from NCBI)


TEXT

For background information on insulin-like growth factor-binding proteins, see 146733.


Cloning and Expression

Kiefer et al. (1991) cloned IGFBP6 from a human osteosarcoma cDNA library. The deduced protein showed 76% sequence identity with IGFBP3 (146732).

Shimasaki et al. (1991) also cloned IGFBP6 and showed that in the human it codes for a deduced 216-amino acid protein with a calculated molecular mass of 22,847 Da. A single 1.3-kb IGFBP6 mRNA was detected by Northern blot analysis in all rat tissues examined, indicating that this binding protein is ubiquitous.


Mapping

Using PCR on human/hamster somatic cell hybrid DNAs, Shimasaki et al. (1991) determined that the IGFBP6 gene is located on chromosome 12.


Gene Function

Kato et al. (1995) showed that the human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.


REFERENCES

  1. Kato, M., Ishizaki, A., Hellman, U., Wernstedt, C., Kyogoku, M., Miyazono, K., Heldin, C.-H., Funa, K. A human keratinocyte cell line produces two autocrine growth inhibitors, transforming growth factor-beta and insulin-like growth factor binding protein-6, in a calcium- and cell density-dependent manner. J. Biol. Chem. 270: 12373-12379, 1995. [PubMed: 7539002, related citations] [Full Text]

  2. Kiefer, M. C., Ioh, R. S., Bauer, D. M., Zapf, J. Molecular cloning of a new human insulin-like growth factor binding protein. Biochem. Biophys. Res. Commun. 176: 219-225, 1991. [PubMed: 1850258, related citations] [Full Text]

  3. Shimasaki, S., Gao, L., Shimonaka, M., Ling, N. Isolation and molecular cloning of insulin-like growth factor-binding protein-6. Molec. Endocr. 5: 938-948, 1991. [PubMed: 1719383, related citations] [Full Text]


Contributors:
Alan F. Scott - updated : 8/8/1995
Creation Date:
Victor A. McKusick : 8/27/1992
carol : 10/04/2006
alopez : 7/21/1998
dkim : 7/2/1998
mark : 11/7/1996
carol : 1/14/1993
carol : 8/27/1992

* 146735

INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 6; IGFBP6


Alternative titles; symbols

IBP6


HGNC Approved Gene Symbol: IGFBP6

Cytogenetic location: 12q13.13     Genomic coordinates (GRCh38): 12:53,097,667-53,102,340 (from NCBI)


TEXT

For background information on insulin-like growth factor-binding proteins, see 146733.


Cloning and Expression

Kiefer et al. (1991) cloned IGFBP6 from a human osteosarcoma cDNA library. The deduced protein showed 76% sequence identity with IGFBP3 (146732).

Shimasaki et al. (1991) also cloned IGFBP6 and showed that in the human it codes for a deduced 216-amino acid protein with a calculated molecular mass of 22,847 Da. A single 1.3-kb IGFBP6 mRNA was detected by Northern blot analysis in all rat tissues examined, indicating that this binding protein is ubiquitous.


Mapping

Using PCR on human/hamster somatic cell hybrid DNAs, Shimasaki et al. (1991) determined that the IGFBP6 gene is located on chromosome 12.


Gene Function

Kato et al. (1995) showed that the human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.


REFERENCES

  1. Kato, M., Ishizaki, A., Hellman, U., Wernstedt, C., Kyogoku, M., Miyazono, K., Heldin, C.-H., Funa, K. A human keratinocyte cell line produces two autocrine growth inhibitors, transforming growth factor-beta and insulin-like growth factor binding protein-6, in a calcium- and cell density-dependent manner. J. Biol. Chem. 270: 12373-12379, 1995. [PubMed: 7539002] [Full Text: https://doi.org/10.1074/jbc.270.21.12373]

  2. Kiefer, M. C., Ioh, R. S., Bauer, D. M., Zapf, J. Molecular cloning of a new human insulin-like growth factor binding protein. Biochem. Biophys. Res. Commun. 176: 219-225, 1991. [PubMed: 1850258] [Full Text: https://doi.org/10.1016/0006-291x(91)90912-q]

  3. Shimasaki, S., Gao, L., Shimonaka, M., Ling, N. Isolation and molecular cloning of insulin-like growth factor-binding protein-6. Molec. Endocr. 5: 938-948, 1991. [PubMed: 1719383] [Full Text: https://doi.org/10.1210/mend-5-7-938]


Contributors:
Alan F. Scott - updated : 8/8/1995

Creation Date:
Victor A. McKusick : 8/27/1992

Edit History:
carol : 10/04/2006
alopez : 7/21/1998
dkim : 7/2/1998
mark : 11/7/1996
carol : 1/14/1993
carol : 8/27/1992