Alternative titles; symbols
HGNC Approved Gene Symbol: MELTF
Cytogenetic location: 3q29 Genomic coordinates (GRCh38): 3:197,001,740-197,029,817 (from NCBI)
The MAP97 gene encodes melanoma-associated antigen p97, a membrane-associated iron-binding protein (summary by Seligman et al., 1986).
P97 is a 97,000-Da cell-surface glycoprotein found in human melanomas. Its amino acid sequence and iron-binding properties are similar to those of transferrin (190000) and lactotransferrin (150210) (summary by Plowman et al., 1983).
Rose et al. (1986) found that the p97 mRNA encodes a 738-residue precursor. They defined domains of the melanotransferrin molecule that showed striking homology to corresponding domains of serum transferrin (39% homology). Conservation of disulfide bridges and other amino acids thought to compose the iron-binding pockets suggested that p97 is also related to transferrin in tissue structure and function.
By study of mouse-man hybrids, Plowman et al. (1983) mapped the gene encoding p97 to chromosome 3. The small amount of p97 produced by fibroblasts could be detected by means of a highly sensitive and specific monoclonal antibody. Of 14 hybrids, 6 contained both chromosome 3 and p97; 8 lacked both.
By in situ hybridization, Yang et al. (1984) mapped P97 to 3q21-q29. By the same method, Le Beau et al. (1986) narrowed the assignment to 3q28-q29. Seligman et al. (1986) found that the p97 antigen is not only functionally related to transferrin receptor (190010) but also maps to the same chromosome region, 3q24-qter. They noted that 3 genes encoding iron-transport proteins, serotransferrin, transferrin receptor, and melanotransferrin, are on chromosome 3.
Le Beau, M. M., Diaz, M. O., Plowman, G. D., Brown, J. P., Rowley, J. D. Chromosomal sublocalization of the human p97 melanoma antigen. Hum. Genet. 72: 294-296, 1986. [PubMed: 3754536] [Full Text: https://doi.org/10.1007/BF00290951]
Plowman, G. D., Brown, J. P., Enns, C. A., Schroder, J., Nikinmaa, B., Sussman, H. H., Hellstrom, K. E., Hellstrom, I. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3. Nature 303: 70-72, 1983. [PubMed: 6843660] [Full Text: https://doi.org/10.1038/303070a0]
Rose, T. M., Plowman, G. D., Teplow, D. B., Dreyer, W. J., Hellstrom, K. E., Brown, J. P. Primary structure of the human melanoma-associated antigen p97 (melanotransferrin) deduced from the mRNA sequence. Proc. Nat. Acad. Sci. 83: 1261-1265, 1986. [PubMed: 2419904] [Full Text: https://doi.org/10.1073/pnas.83.5.1261]
Seligman, P. A., Butler, C. D., Massey, E. J., Kaur, J. A., Brown, J. P., Plowman, G. D., Miller, Y., Jones, C. The p97 antigen is mapped to the q24-qter region of chromosome 3, the same region as the transferrin receptor. Am. J. Hum. Genet. 38: 540-548, 1986. [PubMed: 3010712]
Yang, F., Lum, J. B., McGill, J. R., Moore, C. M., Naylor, S. L., van Bragt, P. H., Baldwin, W. D., Bowman, B. H. Human transferrin: cDNA characterization and chromosomal localization. Proc. Nat. Acad. Sci. 81: 2752-2756, 1984. [PubMed: 6585826] [Full Text: https://doi.org/10.1073/pnas.81.9.2752]