Alternative titles; symbols
Cytogenetic location: 1q23 Genomic coordinates (GRCh38): 1:156,600,001-165,500,000
Jeffreys et al. (1985) described hypervariable minisatellites which have proved to be useful tools for many studies in human molecular genetics. Two classic minisatellite probes, 33.6 and 33.15 (157570), derived from these minisatellite sequences showed a large number (20-30) of highly polymorphic fragments in HinfI digests of human DNA. This extremely polymorphic pattern due to variation in the number of repeats of a 'core' 16-mer sequence allowed the precise identification of a particular individual by his DNA fingerprint. The polymorphic patterns have been used extensively in paternity or sibship testing and in a number of forensic studies. Chimini et al. (1989) observed a single major hybridization peak when either 33.6 or 33.15 was used for in situ hybridization to human metaphase chromosomes, thus permitting mapping to 1q23 and 7q35-q36, respectively. Furthermore, hybridization to human DNA cleaved with 'rare-cutter' enzymes and fractionated on pulsed field gels also showed a fairly simple, largely monomorphic pattern which allowed chromosomal assignment using somatic cell hybrids. Differences in hybridization stringency and degree of resolution account for most of the discrepancy between these results and the accepted view of minisatellites, i.e., that a large number of unlinked loci are spread over the genome.
Chimini, G., Mattei, M.-G., Passage, E., Nguyen, C., Boretto, J., Mattei, J.-F., Jordan, B. R. In situ hybridization and pulsed-field gel analysis define two major minisatellite loci: 1q23 for minisatellite 33.6 and 7q35-q36 for minisatellite 33.15. Genomics 5: 316-324, 1989. [PubMed: 2793185] [Full Text: https://doi.org/10.1016/0888-7543(89)90064-5]
Jeffreys, A. J., Wilson, V., Thein, S. L. Hypervariable 'minisatellite' regions in human DNA. Nature 314: 67-73, 1985. [PubMed: 3856104] [Full Text: https://doi.org/10.1038/314067a0]