Alternative titles; symbols
HGNC Approved Gene Symbol: REG1B
Cytogenetic location: 2p12 Genomic coordinates (GRCh38): 2:79,085,023-79,087,993 (from NCBI)
The REG1A (167770) and REG1B genes belong to the type I subclass of the REG family of genes. Gharib et al. (1993) demonstrated that, in addition to the REG1A gene and the REG pseudogene in the human, there is another sequence, which they named REGL, closely related to the REG1A gene.
Using the sequence of REG1A to screen a human genomic library, Bartoli et al. (1993) identified REG1B, which they designated REGL. The REG1A and REG1B proteins both contain 166 amino acids and differ at only 22 amino acids. REG1B contains 6 half cysteines predicted to form 3 disulfide bridges characteristic of calcium-dependent lectins (see CLEC1A; 606782). RT-PCR detected REG1B expression in pancreas and liver, but not in other tissues examined.
Using probes that did not differentiate between REG1A and REG1B for in situ hybridization and immunohistochemical analysis, Sanchez et al. (2001) found that REG1A and/or REG1B were expressed in acinar cells of human fetal and adult pancreas. RT-PCR analysis using specific primers showed that both transcripts were more highly expressed in adult pancreas than in fetal pancreas. REG1A was the dominant species in fetal samples, and REG1B was the dominant species in adult samples. There did not appear to be developmental regulation of REG1A and REG1B expression between 16 and 41 weeks' gestation.
Bartoli et al. (1993) determined that the REG1B gene contains 6 exons and spans about 3.3 kb. The upstream region contains a TATA box and a CAAT sequence.
Gharib et al. (1993) and Moriizumi et al. (1994) mapped the REG1A and REG1B genes to chromosome 2p12. Miyashita et al. (1995) demonstrated that 4 REG family genes are tandemly ordered in a 95-kb DNA region of 2p12. From analysis of YAC clones containing the 4 genes using 2-color fluorescence in situ hybridization, they demonstrated the following order: 2cen--PAP--RS--REG1A--REG1B--pter. (RS, so designated for REG-related sequence, shows a high degree of homology to the REG1 genes but has an in-frame stop codon in the protein coding region.)
Bartoli, C., Gharib, B., Giorgi, D., Sansonetti, A., Dagorn, J.-C., Berge-Lefranc, J.-L. A gene homologous to the reg gene is expressed in human pancreas. FEBS Lett. 327: 289-293, 1993. [PubMed: 8348956] [Full Text: https://doi.org/10.1016/0014-5793(93)81006-l]
Gharib, B., Fox, M. F., Bartoli, C., Giorgi, D., Sansonetti, A., Swallow, D. M., Dagorn, J. C., Berge-Lefranc, J. L. Human regeneration protein/lithostathine genes map to chromosome 2p12. Ann. Hum. Genet. 57: 9-16, 1993. [PubMed: 8333731] [Full Text: https://doi.org/10.1111/j.1469-1809.1993.tb00882.x]
Miyashita, H., Nakagawara, K., Mori, M., Narushima, Y., Noguchi, N., Moriizumi, S., Takasawa, S., Yonekura, H., Takeuchi, T., Okamoto, H. Human REG family genes are tandemly ordered in a 95-kilobase region of chromosome 2p12. FEBS Lett. 377: 429-433, 1995. [PubMed: 8549770] [Full Text: https://doi.org/10.1016/0014-5793(95)01381-4]
Moriizumi, S., Watanabe, T., Unno, M., Nakagawara, K., Suzuki, Y., Miyashita, H., Yonekura, H., Okamoto, H. Isolation, structural determination and expression of a novel reg gene, human regI-beta. Biochim. Biophys. Acta 1217: 199-202, 1994. [PubMed: 8110835] [Full Text: https://doi.org/10.1016/0167-4781(94)90035-3]
Sanchez, D., Figarella, C., Marchand-Pinatel, S., Bruneau, N., Guy-Crotte, O. Preferential expression of Reg I-beta gene in human adult pancreas. Biochem. Biophys. Res. Commun. 284: 729-737, 2001. [PubMed: 11396963] [Full Text: https://doi.org/10.1006/bbrc.2001.5033]