Alternative titles; symbols
HGNC Approved Gene Symbol: PLGLB1
Cytogenetic location: 2p11.2 Genomic coordinates (GRCh38): 2:87,010,224-87,021,844 (from NCBI)
Using a partial plasminogen (PLG; 173350) sequence to screen a human fetal liver cDNA library, Lewis et al. (1999) cloned PLGLB1, which they called PRGB. The deduced 96-amino peptide has a calculated molecular mass of 11.2 kD. PRGB has signal sequence followed by a 77-amino acid stretch that is identical to the N terminus of plasminogen and highly similar to the homologous stretch in PRGA (PLGLA; 612212). PRGB is predicted to have 2 disulfide bonds and assume a secondary structure including an alpha helix and antiparallel beta sheet. Northern blot analysis using a probe that did not differentiate between PRGA and PRGB detected PRG only in liver and in a lymphoblastic leukemia cell line.
Kida et al. (1997) characterized the 5-prime flanking region of the human PLGLB1 gene and found 3 TATA boxes 550 to 600 bp upstream of the transcription initiation site, a TATA-like sequence (TGTAA) at position -16, and putative binding sites for several transcription factors. The 1.1-kb 5-prime flanking sequence directed basal liver-specific expression in HepG2 cells, and deletion analysis identified positive and negative elements in the PLGLB1 promoter.
Frank et al. (1989) used a plasminogen probe encoding kringles 1-3 to detect homologous loci on human chromosomes 6 and 2 by somatic cell hybrid analysis. Regional localization by in situ hybridization placed the loci at 6q26-q27 and 2p11-q11. Further analysis by varying washing stringencies of hybridization filters showed greater homology with the chromosome 6 locus than with the chromosome 2 locus. The results were interpreted as confirming localization of the PLG gene to chromosome 6 and indicating that a homologous sequence of unknown identity is located on chromosome 2. The sequence on chromosome 2 may represent a pseudogene or it may be an unidentified kringle-containing protein. Magnaghi et al. (1995) presented a restriction map showing the orientation of the PLG and PLGL genes to each other and to the LPA gene (152200) and the LPA-like gene.
Frank, S. L., Klisak, I., Sparkes, R. S., Lusis, A. J. A gene homologous to plasminogen located on human chromosome 2q11-p11. Genomics 4: 449-451, 1989. [PubMed: 2714803] [Full Text: https://doi.org/10.1016/0888-7543(89)90356-x]
Kida, M., Wakabayashi, S., Ichinose, A. Characterization of the 5-prime-flanking regions of plasminogen-related genes A and B. FEBS Lett. 404: 95-99, 1997. [PubMed: 9074645] [Full Text: https://doi.org/10.1016/s0014-5793(97)00101-4]
Lewis, V. O., Gehrmann, M., Weissbach, L., Hyman, J. E., Rielly, A., Jones, D. G., Llinas, M., Schaller, J. Homologous plasminogen N-terminal and plasminogen-related gene A and B peptides: characterization of cDNAs and recombinant fusion proteins. Europ. J. Biochem. 259: 618-625, 1999. [PubMed: 10092845] [Full Text: https://doi.org/10.1046/j.1432-1327.1999.00055.x]
Magnaghi, P., Agazzi, A., Semino, O., Ferrari, M., Barbui, T., D'Angelo, A., Taramelli, R. A recombination event in the closely linked plasminogen and apolipoprotein(a) gene loci. Clin. Genet. 47: 285-289, 1995. [PubMed: 7554360] [Full Text: https://doi.org/10.1111/j.1399-0004.1995.tb03966.x]