Alternative titles; symbols
HGNC Approved Gene Symbol: RBP1
Cytogenetic location: 3q23 Genomic coordinates (GRCh38): 3:139,517,438-139,539,742 (from NCBI)
Retinol-binding protein-4 (RBP4; 180250) is the carrier protein involved in the transport of retinol from the liver storage site to peripheral tissue. On the other hand, cellular RBP (CRBP) is the intracellular carrier involved in intracellular movement of retinol.
Colantuoni et al. (1985) isolated and sequenced a cDNA clone for the human CRBP gene. Nilsson et al. (1988) stated that CRBP is an acidic 132-amino acid protein with a molecular mass of 15.7 kD.
Kawaguchi et al. (2007) identified STRA6 (610745) as the membrane receptor for RBP1, which mediates cellular uptake of vitamin A.
Zizola et al. (2010) found that mouse 3T3-L1 preadipocytes upregulated Crbp1 expression early during differentiation, and downregulated Crbp1 expression thereafter. Knockdown of Crbp1 via small hairpin RNA enhanced lipid accumulation, elevated Ppar-gamma (PPARG; 601487) activity, and enhanced adipocyte differentiation. Embryonic fibroblasts cultured from Crbp1-knockout mice showed similar changes. Reexpression of Crbp1 in Crbp1 knockdown 3T3-L1 cells or Crbp1-knockout fibroblasts normalized intracellular triglyceride levels. Manipulation of Crbp1 expression did not alter the intracellular levels of retinaldehyde or retinoic acid (RA) or alter expression of RA target genes. Zizola et al. (2010) concluded that Crbp1 is a negative regulator of Ppar-gamma activity.
Nilsson et al. (1988) determined that the CRBP gene contains 4 exons encoding 24, 59, 33, and 16 amino acid residues, respectively. The second intron alone occupies 19 kb of the 21 kb of the CRBP gene.
By somatic cell hybrid analysis, Colantuoni et al. (1986) assigned the CRBP gene to chromosome 3. Nilsson et al. (1988) assigned the CRBP gene to 3p11-qter using hybridization to mouse-human, rat-human, and hamster-human cell hybrids. By combination of in situ hybridization and Southern blots of genomic DNA from hybrid cells, Rocchi et al. (1989) narrowed the assignment of RBP1 to 3q21-q22. De Baere et al. (1998) refined the localization to 3q23 by FISH and Southern blot analysis.
Zizola et al. (2010) found that body masses of Crbp1 -/- and wildtype mice were indistinguishable when both were fed a high-fat diet, but that Crbp1 -/- mice exhibited increased adiposity compared with wildtype. Crbp1 -/- mice were more glucose tolerant and more sensitive to insulin than wildtype mice during diet-induced obesity. White adipose tissue of Crbp1 -/- mice showed elevated expression of Ppar-gamma target genes.
Kane et al. (2011) found that Rbp1 -/- mice showed a compensatory increase in Rbp2 (180280) and Crabp2 (180231) expression and elevated pancreatic content of activated RA, 9-cis-RA (9cRA). Elevated 9cRA signaling disrupted glucose sensing and impaired insulin secretion, but it enhanced glucagon secretion after feeding. Rbp1 -/- mice were hyperglycemic, relied on increased fatty acid oxidation, and resisted diet-induced obesity.
Colantuoni, V., Cortese, R., Nilsson, M., Lundvall, J., Bavik, C. O., Eriksson, U., Peterson, P. A., Sundelin, J. Cloning and sequencing of a full length cDNA corresponding to human cellular retinol-binding protein. Biochem. Biophys. Res. Commun. 130: 431-439, 1985. [PubMed: 2992469] [Full Text: https://doi.org/10.1016/0006-291x(85)90435-8]
Colantuoni, V., Rocchi, M., Roncuzzi, L., Romeo, G. Mapping of human cellular retinol-binding protein to chromosome 3. Cytogenet. Cell Genet. 43: 221-222, 1986. [PubMed: 3802925] [Full Text: https://doi.org/10.1159/000132326]
De Baere, E., Speleman, F., Van Roy, N., De Paepe, A., Messiaen, L. Assignment of the cellular retinol-binding protein 1 gene (RBP1) and of the coatomer beta-prime subunit gene (COPB2) to human chromosome band 3q23 by in situ hybridization. Cytogenet. Cell Genet. 82: 226-227, 1998. [PubMed: 9858824] [Full Text: https://doi.org/10.1159/000015107]
Kane, M. A., Folias, A. E., Pingitore, A., Perri, M., Krois, C. R., Ryu, J. Y., Cione, E., Napoli, J. L. CrbpI modulates glucose homeostasis and pancreas 9-cis-retinoic acid concentrations. Molec. Cell. Biol. 31: 3277-3285, 2011. [PubMed: 21670153] [Full Text: https://doi.org/10.1128/MCB.05516-11]
Kawaguchi, R., Yu, J., Honda, J., Hu, J., Whitelegge, J., Ping, P., Wiita, P., Bok, D., Sun, H. A membrane receptor for retinol binding protein mediates cellular uptake of vitamin A. Science 315: 820-825, 2007. [PubMed: 17255476] [Full Text: https://doi.org/10.1126/science.1136244]
Nilsson, M. H. L., Spurr, N. K., Lundvall, J., Rask, L., Peterson, P. A. Human cellular retinol-binding protein gene organization and chromosomal location. Europ. J. Biochem. 173: 35-44, 1988. [PubMed: 3356192] [Full Text: https://doi.org/10.1111/j.1432-1033.1988.tb13963.x]
Rocchi, M., Covone, A., Romeo, G., Faraonio, R., Colantuoni, V. Regional mapping of RBP4 to 10q23-q24 and RBP1 to 3q21-q22 in man. Somat. Cell Molec. Genet. 15: 185-190, 1989. [PubMed: 2928844] [Full Text: https://doi.org/10.1007/BF01535081]
Zizola, C. F., Frey, S. K., Jitngarmkusol, S., Kadereit, B., Yan, N., Vogel, S. Cellular retinol-binding protein type I (CRBP-I) regulates adipogenesis. Molec. Cell. Biol. 30: 3412-3420, 2010. [PubMed: 20498279] [Full Text: https://doi.org/10.1128/MCB.00014-10]