Alternative titles; symbols
HGNC Approved Gene Symbol: RPLP0
Cytogenetic location: 12q24.23 Genomic coordinates (GRCh38): 12:120,196,699-120,201,111 (from NCBI)
Acidic ribosomal proteins, called A-proteins (acidic) or P-proteins (phosphorylated acidic), such as RPLP0, are generally present in multiple copies on the ribosome and have isoelectric points in the range of pH 3 to 5, in contrast with most ribosomal proteins, which are single copy and basic. A-proteins have hydrophobic amino acid compositions, notably about 20% alanine (summary by Rich and Steitz, 1987).
Rich and Steitz (1987) described the isolation and analysis of 3 cDNA molecules that encode the human P-proteins, P2 (180530), P1 (180520), and P0. The human P1 and P2 cDNA nucleotide sequences and deduced amino acid sequences of the proteins are very similar to the sequences that have been determined for the corresponding rat, shrimp, and yeast proteins and cDNAs. P0 sequences had not previously been described. To demonstrate that the coding sequences are full length, Rich and Steitz (1987) transcribed the P0, P1, and P2 cDNAs in vitro. The P0, P1, and P2 proteins produced were serologically and electrophoretically identical to P-proteins extracted from HeLa cells. The deduced P0 protein contains 317 amino acids and is the largest of the 3 proteins. Northern blot analysis revealed expression of a 1.2-kb transcript in HeLa cells.
Using yeast 2-hybrid analysis, Zou et al. (2003) found that VCY (400012) interacted with acidic ribosomal protein P0.
Hartz (2016) mapped the RPLP0 gene to chromosome 12q24.23 based on an alignment of the RPLP0 sequence (GenBank AF274958) with the genomic sequence (GRCh38).
Hartz, P. A. Personal Communication. Baltimore, Md. 8/25/2016.
Rich, B. E., Steitz, J. A. Human acidic ribosomal phosphoproteins P0, P1, and P2: analysis of cDNA clones, in vitro synthesis, and assembly. Molec. Cell. Biol. 7: 4065-4074, 1987. [PubMed: 3323886] [Full Text: https://doi.org/10.1128/mcb.7.11.4065-4074.1987]
Zou, S. W., Zhang, J. C., Zhang, X. D., Miao, S. Y., Zong, S. D., Sheng, Q., Wang, L. F. Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis. Cell Res. 13: 171-177, 2003. [PubMed: 12862317] [Full Text: https://doi.org/10.1038/sj.cr.7290161]