Alternative titles; symbols
HGNC Approved Gene Symbol: SRM
Cytogenetic location: 1p36.22 Genomic coordinates (GRCh38): 1:11,054,589-11,060,018 (from NCBI)
The biosynthesis of polyamines, which are universally essential for cellular functions, takes place from arginine and methionine and involves 4 distinct enzymes: spermidine synthase (EC 2.5.1.16), ornithine decarboxylase (165640), S-adenosyl-L-methionine decarboxylase (180980), and spermine synthase (300105). Wahlfors et al. (1990) cloned a cDNA coding for the full-length subunit of spermidine synthase, using a synthetic deoxyoligonucleotide mixture corresponding to a tryptic peptide of the bovine enzyme and a human decidual cDNA library. An open reading frame for a 302-amino acid polypeptide was found.
Myohanen et al. (1991) isolated the SRM gene from a genomic library and showed that it comprises 5,818 nucleotides from the cap site to the last A of the putative polyadenylation signal with 8 exons.
Using Southern blot analysis of panels of human/rodent somatic cell hybrids and chromosomal in situ hybridization, Winqvist et al. (1991) assigned spermidine synthase DNA sequences to 1p36-p22 and 3p14-q21. As determined from the intensity of the hybridization signal observed in analysis of DNA from somatic cell hybrids, it seemed likely that the functional gene is located at the former site.
By PCR analysis of DNA obtained from human/hamster somatic cell hybrids, Myohanen et al. (1991) mapped the SRM gene to chromosome 1.
Myohanen, S., Kauppinen, L., Wahlfors, J., Alhonen, L., Janne, J. Human spermidine synthase gene: structure and chromosomal localization. DNA Cell Biol. 10: 467-474, 1991. [PubMed: 2069720] [Full Text: https://doi.org/10.1089/dna.1991.10.467]
Wahlfors, J., Alhonen, L., Kauppinen, L., Hyvonen, T., Janne, J., Eloranta, T. O. Human spermidine synthase: cloning and primary structure. DNA Cell Biol. 9: 103-110, 1990. Note: Erratum: DNA Cell Biol. 9: 543 only, 1990. [PubMed: 2344393] [Full Text: https://doi.org/10.1089/dna.1990.9.103]
Winqvist, R., Alanen, L., Grzeschik, K.-H., Janne, J., Eloranta, T. Mapping of DNA sequences for spermidine synthase to human chromosome 1p36-p22 and chromosome 3p14-q21. (Abstract) Cytogenet. Cell Genet. 58: 1865 only, 1991.