Alternative titles; symbols
HGNC Approved Gene Symbol: CRISP2
Cytogenetic location: 6p12.3 Genomic coordinates (GRCh38): 6:49,676,652-49,714,220 (from NCBI)
In the course of characterizing a large number of genomic clones isolated from mouse chromosome 17, Kasahara et al. (1987) found a cosmid clone containing a sequence transcribed specifically in the testis. Subsequent studies (Kasahara et al., 1989) demonstrated a map location between Pgk2 and Mep1 on mouse chromosome 17, making it unlikely that the gene is involved in t mutant phenotypes. Kasahara et al. (1989) described the cDNA sequences of the mouse gene, named Tpx1, and of its human counterpart TPX1. The 2 showed 77.8% nucleotide and 70% amino acid sequence similarity. The mouse gene showed 64.2% nucleotide and 55.1% amino acid sequence similarity to that of the rat sperm-coating glycoprotein gene (Scg), the protein product of which is secreted by the epididymis.
Using a yeast 2-hybrid assay, Jamsai et al. (2008) showed that mouse Crisp2 bound to the Ggn (609966) splice variant Ggn1, but not to Ggn2 or Ggn3. Ggn1 protein colocalized with Crisp2 in the principal piece of the sperm tail.
By Southern blot analysis of somatic cell hybrid DNA, Kasahara et al. (1989) mapped the CRISP2 gene to chromosome 6p21-qter.
Stumpf (2022) mapped the CRISP2 gene to chromosome 6p12.3 based on an alignment of the CRISP2 sequence (GenBank BC107707.1) with the genomic sequence (GRCh38).
Jamsai, D., Bianco, D. M., Smith, S. J., Merriner, D. J., Ly-Huynh, J. D., Herlihy, A., Niranjan, B., Gibbs, G. M., O'Bryan, M. K. Characterization of gametogenetin 1 (GGN1) and its potential role in male fertility through the interaction with the ion channel regulator, cysteine-rich secretory protein 2 (CRISP2) in the sperm tail. Reproduction 135: 751-759, 2008. [PubMed: 18502891] [Full Text: https://doi.org/10.1530/REP-07-0485]
Kasahara, M., Figueroa, F., Klein, J. Random cloning of genes from mouse chromosome 17. Proc. Nat. Acad. Sci. 84: 3325-3328, 1987. [PubMed: 3472212] [Full Text: https://doi.org/10.1073/pnas.84.10.3325]
Kasahara, M., Gutknecht, J., Brew, K., Spurr, N., Goodfellow, P. N. Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene. Genomics 5: 527-534, 1989. [PubMed: 2613236] [Full Text: https://doi.org/10.1016/0888-7543(89)90019-0]
Kasahara, M., Passmore, H. C., Klein, J. A testis-specific gene Tpx-1 maps between Pgk-2 and Mep-1 on mouse chromosome 17. Immunogenetics 29: 61-63, 1989. [PubMed: 2562800] [Full Text: https://doi.org/10.1007/BF02341616]
Stumpf, A. M. Personal Communication. Baltimore, Md. 04/06/2022.