Alternative titles; symbols
HGNC Approved Gene Symbol: GTF2F1
Cytogenetic location: 19p13.3 Genomic coordinates (GRCh38): 19:6,379,572-6,393,164 (from NCBI)
The GTF2F1 gene encodes the alpha subunit of the general transcription factor TFIIF. TFIIF acts after TFIID (313650) firmly associates with DNA, but coincidentally with or immediately after RNA polymerase II binding to DNA, and before the recruitment of factor TFIIE (189962, 189964) (summary by Aso et al., 1992).
Aso et al. (1992) partially sequenced the RAP74 protein from purified HeLa cells, cloned its cDNA, and showed that its translation product can interact with RAP30 (189969) in vitro as well as in vivo. The cDNA predicted an amino acid sequence that lacks obvious DNA or RNA helicase motifs. The small subunit (RAP30) of TFIIF was cloned by Sopta et al. (1989) and shown to have some amino acid sequence homology to bacterial sigma factors.
Finkelstein et al. (1992) likewise isolated a cDNA encoding RAP74 and showed that both RAP30 and RAP74 produced in Escherichia coli could be used in place of natural human RAP30/74 to direct accurate transcription initiation by RNA polymerase II in vitro.
Joliot et al. (1995) demonstrated that interaction with the RAP74 subunit of TFIIF is necessary for transcriptional activation by serum response factor (600246).
By in situ hybridization, Aso et al. (1993) mapped the GTF2F1 gene to chromosome 19p13.3.
Aso, T., Tsai, P., Kawaguchi, T., Menninger, J. C., Kitajima, S., Yasukochi, Y., Ward, D. C., Weissman, S. M. Assignment of the human GTF2F1 gene to chromosome 19p13.3. Genomics 16: 252-253, 1993. [PubMed: 8486367] [Full Text: https://doi.org/10.1006/geno.1993.1168]
Aso, T., Vasavada, H. A., Kawaguchi, T., Germino, F. J., Ganguly, S., Kitajima, S., Weissman, S. M., Yasukochi, Y. Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF. Nature 355: 461-464, 1992. [PubMed: 1734283] [Full Text: https://doi.org/10.1038/355461a0]
Finkelstein, A., Kostrub, C. F., Li, J., Chavez, D. P., Wang, B. Q., Fang, S. M., Greenblatt, J., Burton, Z. F. A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II. Nature 355: 464-467, 1992. [PubMed: 1734284] [Full Text: https://doi.org/10.1038/355464a0]
Joliot, V., Demma, M., Prywes, R. Interaction with RAP74 subunit of TFIIF is required for transcriptional activation by serum response factor. Nature 373: 632-635, 1995. [PubMed: 7854423] [Full Text: https://doi.org/10.1038/373632a0]
Sopta, M., Burton, Z. F., Greenblatt, J. Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II. Nature 341: 410-414, 1989. [PubMed: 2477704] [Full Text: https://doi.org/10.1038/341410a0]