Alternative titles; symbols
HGNC Approved Gene Symbol: ZBTB25
Cytogenetic location: 14q23.3 Genomic coordinates (GRCh38): 14:64,449,106-64,505,213 (from NCBI)
Chardin et al. (1991) isolated a human cDNA encoding a protein with 2 zinc fingers in its C-terminal part, which they named KUP for Kruppel-related protein. Genomic sequences hybridizing with the human KUP probe were found in rat and mouse. The highest levels of expression of the gene were found in testis, fetal liver, and hematopoietic cells. The KUP protein is 433 amino acids long, has a molecular weight of about 50 kD, and binds to DNA. Its structure, resembling that of the Kruppel family, suggests that it is also a transcription factor.
Using a systems analysis of transcriptional regulators within T cells during different phases of development and differentiation, Benita et al. (2010) identified ZBTB25, a BTB-POZ family transcription factor, as a highly T cell-enriched transcription factor. They provided evidence that ZBTB25 negatively regulates NFAT (see 600489) activation, including RNA interference studies showing ZBTB25 knockdown resulted in enhanced activation of NFAT target genes after T-cell receptor engagement. Benita et al. (2010) concluded that ZBTB25 has a role in NFAT-mediated gene expression.
By in situ hybridization, Chardin et al. (1991) assigned the ZNF46 gene to chromosome 14q23-q24.
Benita, Y., Cao, Z., Giallourakis, C., Li, C., Gardet, A., Xavier, R. J. Gene enrichment profiles reveal T-cell development, differentiation, and lineage-specific transcription factors including ZBTB25 as a novel NF-AT repressor. Blood 115: 5376-5384, 2010. [PubMed: 20410506] [Full Text: https://doi.org/10.1182/blood-2010-01-263855]
Chardin, P., Courtois, G., Mattei, M.-G., Gisselbrecht, S. The KUP gene, located on human chromosome 14, encodes a protein with two distant zinc fingers. Nucleic Acids Res. 19: 1431-1436, 1991. [PubMed: 2027750] [Full Text: https://doi.org/10.1093/nar/19.7.1431]