Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF45
Cytogenetic location: 19q13.31 Genomic coordinates (GRCh38): 19:43,912,624-43,935,282 (from NCBI)
Zinc finger genes represent a large family of more than 500 genes in the human genome that are involved in transcription regulation. A subgroup of these genes contains a conserved motif, the KRAB domain (see 601742), at the N-terminal region. The KRAB domain possesses a potent transcriptional repression activity. Constantinou Deltas et al. (1996) determined the complete 2,046-bp coding sequence for one such gene, ZNF45. The predicted protein product contains 682 amino acids.
Shannon and Stubbs (1998) found that the predicted ZNF45 protein contains KRAB A and B domains, a spacer region, and 17 tandemly arrayed zinc fingers of which 2 are degenerate. Northern blot analysis of adult human tissues detected a 3.5-kb ZNF45 transcript in many tissues including spleen, thymus, blood leukocytes, pancreas, small intestine, colon, heart, brain, skeletal muscle, testis, prostate, ovary, and placenta.
Constantinou Deltas et al. (1996) determined the exon/intron arrangement and splice junctions of the ZNF45 gene. Exon 2 codes exclusively for the KRAB-A element of 42 amino acids, and exon 4 contains 19 zinc finger repeats.
By analysis of somatic cell hybrids and by in situ hybridization, Rousseau-Merck et al. (1993) demonstrated that the KOX22 (ZNF224; 194555) gene and the KOX5 (ZNF45) genes map to 19q13.2-qter. Furthermore, by pulsed field gel electrophoresis experiments they showed that the pair of genes lie within a DNA segment less than 300 kb long.
Constantinou Deltas et al. (1996) generated an expressed sequence tag from the 3-prime end of ZNF45 and used it for PCR screening of a P1-derived genomic library. In this way they isolated a 65-kb clone that was used for localizing the gene on 19q13.2 by fluorescence in situ hybridization.
By PCR, Shannon and Stubbs (1998) localized the ZNF45 gene to a specific cosmid within a 19q13.2 cosmid contig.
Constantinou Deltas, C., Bashiardes, E., Patsalis, P. C., Hadjimarcou, M., Kroisel, P. M., Ioannou, P. A., Roses, A. D., Lee, J. E. Complete coding sequence, exon/intron arrangement and chromosome location of ZNF45, a KRAB-domain-containing gene. Cytogenet. Cell Genet. 75: 230-233, 1996. [PubMed: 9067431] [Full Text: https://doi.org/10.1159/000134490]
Rousseau-Merck, M.-F., Hillion, J., Jonveaux, P., Couillin, P., Seite, P., Thiesen, H.-J., Berger, R. Chromosomal localization of 9 KOX zinc finger genes: physical linkages suggest clustering of KOX genes on chromosomes 12, 16, and 19. Hum. Genet. 92: 583-587, 1993. [PubMed: 8262519] [Full Text: https://doi.org/10.1007/BF00420943]
Shannon, M., Stubbs, L. Analysis of homologous XRCC1-linked zinc-finger gene families in human and mouse: evidence for orthologous genes. Genomics 49: 112-121, 1998. [PubMed: 9570955] [Full Text: https://doi.org/10.1006/geno.1998.5230]