Alternative titles; symbols
HGNC Approved Gene Symbol: PCOLCE
Cytogenetic location: 7q22.1 Genomic coordinates (GRCh38): 7:100,602,363-100,608,175 (from NCBI)
Type I procollagen COOH-terminal proteinase enhancer is a glycoprotein that binds to the COOH-terminal propeptide of type I procollagen and enhances C-proteinase activity (Takahara et al., 1994).
Takahara et al. (1994) purified type I procollagen COOH-terminal proteinase enhancer (Pcolce) from mouse fibroblast culture medium. Sequences of mouse enhancer cDNA predicted a 468-amino acid protein. Takahara et al. (1994) noted that in the mouse, procollagen C-proteinase depends for full expression upon the presence of either a 55-kD glycoprotein or smaller 36- and 34-kD proteolytically processed forms of the same protein. They showed that the 36-kD form corresponds to the N-terminal portion of the 55-kD form, which is composed almost exclusively of 2 CUB domains. Mouse enhancer RNA was shown to be at highest levels in collagen-rich tissues, especially tendon.
Takahara et al. (1994) cloned human PCOLCE from a placenta cDNA library. The deduced 449-amino acid protein shares high sequence homology with the mouse protein, particularly in the CUB domains in the N terminus.
By Southern analysis of genomic DNA from panels of human/mouse cell hybrids and by fluorescence in situ hybridization, Takahara et al. (1994) localized the PCOLCE gene to 7q21.3-q22, the same region containing the type I collagen alpha-2 chain gene (COL1A2; 120160). Takahara et al. (1996) investigated further the apparent proximity of the 2 genes by the study of somatic cell hybrids, cosmid contigs, and interspecific backcross mice. They found that PCOLCE and COL1A2 are separated by at least 6 Mb and that Pcolce and Col1a2 are located on separate chromosomes in the mouse, chromosomes 5 and 6, respectively.
Takahara, K., Kessler, E., Biniaminov, L., Brusel, M., Eddy, R. L., Jani-Sait, S., Shows, T. B., Greenspan, D. S. Type I procollagen COOH-terminal proteinase enhancer protein: identification, primary structure, and chromosomal localization of the cognate human gene (PCOLCE). J. Biol. Chem. 269: 26280-26285, 1994. [PubMed: 7523404]
Takahara, K., Osborne, L., Elliott, R. W., Tsui, L.-C., Scherer, S. W., Greenspan, D. S. Fine mapping of the human and mouse genes for the type I procollagen COOH-terminal proteinase enhancer protein. Genomics 31: 253-256, 1996. [PubMed: 8824813] [Full Text: https://doi.org/10.1006/geno.1996.0043]