HGNC Approved Gene Symbol: RPS8
Cytogenetic location: 1p34.1 Genomic coordinates (GRCh38): 1:44,775,540-44,778,779 (from NCBI)
The eukaryotic ribosome is composed of 4 RNA molecules (see 180450) and approximately 80 proteins. Davies and Fried (1993) used a PCR-based cloning strategy to isolate the intron-containing gene encoding human ribosomal protein S8 in the presence of its many processed pseudogenes and determined the DNA sequence (GenBank X67247) of the entire gene, as well as of its upstream and downstream flanking regions. The RPS8 gene is 3,161 bp long.
By Northern blot analysis using a rat Rps8 probe, Pogue-Geile et al. (1991) found increased levels of RPS8 mRNA in all 3 human colorectal tumors and all 3 human colon polyps examined, as compared to matched normal colonic mucosa. RPS8 is expressed as an approximately 980-bp transcript.
Davies and Fried (1993) determined that the RPS8 gene compromises 6 exons. Despite lacking a consensus TATA box, primer extension analysis indicated that the start of transcription is precisely located at a C residue within an 11-bp oligopyrimidine tract. The first exon, which contains the ATG start codon, is only 27 bp long.
Davies and Fried (1993) localized the human RPS8 gene to 1p34.1-p32 using a combination of Southern blotting, PCR, and fluorescence in situ hybridization. Kenmochi et al. (1998) confirmed the mapping assignment reported by Davies and Fried (1993).
Davies, B., Fried, M. The structure of the human intron-containing S8 ribosomal protein gene and determination of its chromosomal location at 1p32-p34.1. Genomics 15: 68-75, 1993. [PubMed: 8432552] [Full Text: https://doi.org/10.1006/geno.1993.1011]
Kenmochi, N., Kawaguchi, T., Rozen, S., Davis, E., Goodman, N., Hudson, T. J., Tanaka, T., Page, D. C. A map of 75 human ribosomal protein genes. Genome Res. 8: 509-523, 1998. [PubMed: 9582194] [Full Text: https://doi.org/10.1101/gr.8.5.509]
Pogue-Geile, K., Geiser, J. R., Shu, M., Miller, C., Wool, I. G., Meisler, A. I., Pipas, J. M. Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein. Molec. Cell. Biol. 11: 3842-3849, 1991. [PubMed: 1712897] [Full Text: https://doi.org/10.1128/mcb.11.8.3842-3849.1991]