Alternative titles; symbols
HGNC Approved Gene Symbol: CSTF3
Cytogenetic location: 11p13 Genomic coordinates (GRCh38): 11:33,084,584-33,161,480 (from NCBI)
Polyadenylation of mRNA precursors is a complex process that requires multiple protein factors, including cleavage stimulation factor (CSTF), a cleavage-polyadenylation specificity factor (see 603052), 2 cleavage factors, and poly(A)+ polymerase. CSTF is composed of 3 subunits: CSTF1 (600369), CSFT2 (300907), and CSTF3 (Takagaki and Manley, 1994).
Takagaki and Manley (1994) cloned human CSTF3, which encodes a 77-kD protein homologous to Drosophila 'suppressor of forked' (suf) and yeast Rna14, both of which are involved in mRNA processing.
By searching for genes in a region of chromosome 11 associated with WAGR syndrome (194072), Gawin et al. (1999) identified and cloned CSTF3. Northern blot analysis of several human tissues detected a 3.2-kb transcript predominantly in lung. In mouse, Cstf3 expression was strong during embryonic development beginning at embryonic day 11 and much weaker in adult tissues.
Takagaki and Manley (2000) reported that human CSTF77 contains 8 tetratricopeptide repeat (TPR) motifs in its N-terminal half, followed by a central nuclear localization signal, 3 additional TPR motifs, and a proline-rich region near the C terminus.
By analysis of a PAC contig covering chromosome 11p14.1-p13, Gawin et al. (1999) mapped the CSTF3 gene to chromosome 11p13, centromeric to the TR2 gene (DEPDC7; 612294) and telomeric to the G2 gene (C11ORF41; 612297).
Gawin, B., Niederfuhr, A., Schumacher, N., Hummerich, H., Little, P. F. R., Gessler, M. A 7.5 Mb sequence-ready PAC contig and gene expression map of human chromosome 11p13-p14.1. Genome Res. 9: 1074-1086, 1999. [PubMed: 10568747] [Full Text: https://doi.org/10.1101/gr.9.11.1074]
Takagaki, Y., Manley, J. L. Complex protein interactions within the human polyadenylation machinery identify a novel component. Molec. Cell. Biol. 20: 1515-1525, 2000. [PubMed: 10669729] [Full Text: https://doi.org/10.1128/MCB.20.5.1515-1525.2000]
Takagaki, Y., Manley, J. L. A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein. Nature 372: 471-474, 1994. [PubMed: 7984242] [Full Text: https://doi.org/10.1038/372471a0]