HGNC Approved Gene Symbol: CSTF1
Cytogenetic location: 20q13.2-q13.31 Genomic coordinates (GRCh38): 20:56,392,379-56,406,362 (from NCBI)
Polyadenylation of mRNA is a complex process that requires multiple protein factors, including 3 cleavage stimulation factors (CSTF1; CSFT2, 300907; and CSTF3, 600367). CstF1, a 50-kD protein cloned by Takagaki and Manley (1992), contains transducin-like repeats characteristic of mammalian G protein beta subunits.
Kleiman and Manley (1999) demonstrated that CSTF1 interacts in vitro and in intact cells with BARD1 (601593). The BARD1-CSTF1 interaction inhibited polyadenylation in vitro. BARD1, like CSTF1, interacts with RNA polymerase-2. BARD1, BRCA1, and CSTF1 were shown to associate in vivo. Kleiman and Manley (1999) demonstrated that BARD1 inhibits pre-mRNA 3-prime cleavage in vitro and that the same region of BARD1 required for binding of CSTF1 is necessary for inhibiting 3-prime pre-mRNA cleavage. Kleiman and Manley (1999) concluded that their results suggested a model in which BARD1, as part of the RNA polymerase-2 holoenzyme, senses sites of DNA damage and repair, and the inhibitory interaction with CSTF1 ensures that nascent RNAs are not erroneously polyadenylated at such sites.
Kleiman, F. E., Manley, J. L. Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50. Science 285: 1576-1579, 1999. [PubMed: 10477523] [Full Text: https://doi.org/10.1126/science.285.5433.1576]
Takagaki, Y., Manley, J. L. A human polyadenylation factor is a G protein beta-subunit homologue. J. Biol. Chem. 267: 23471-23474, 1992. [PubMed: 1358884]