Alternative titles; symbols
HGNC Approved Gene Symbol: RFC3
Cytogenetic location: 13q13.2 Genomic coordinates (GRCh38): 13:33,818,149-33,977,377 (from NCBI)
See replication factor C, subunit 2 (RFC2; 600404).
Crystal Structure
Bowman et al. (2004) reported the crystal structure of the 5-protein clamp loader complex (replication factor-C, RFC) of the yeast S. cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, or PCNA; 176740). Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring. Placement of a model for primed DNA within the central hole of PCNA revealed a striking correspondence between the RFC spiral and the grooves of the DNA double helix. Bowman et al. (2004) concluded that this model, in which the clamp loader complex locks into primed DNA in a screwcap-like arrangement, provides a simple explanation for the process by which the engagement of primer-template junctions by the RFC:PCNA complex results in ATP hydrolysis and release of the sliding clamp on DNA.
Okumura et al. (1995) mapped the RFC3 gene to 13q12.3-q13 by PCR amplification of DNAs from a panel of somatic hybrids and by fluorescence in situ hybridization.
Bowman, G. D., O'Donnell, M., Kuriyan, J. Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex. Nature 429: 724-730, 2004. [PubMed: 15201901] [Full Text: https://doi.org/10.1038/nature02585]
Okumura, K., Nogami, M., Taguchi, H., Dean, F. B., Chen, M., Pan, Z.-Q., Hurwitz, J., Shiratori, A., Murakami, Y., Ozawa, K., Eki, T. Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23. Genomics 25: 274-278, 1995. [PubMed: 7774928] [Full Text: https://doi.org/10.1016/0888-7543(95)80135-9]