Alternative titles; symbols
HGNC Approved Gene Symbol: TAF7
Cytogenetic location: 5q31.3 Genomic coordinates (GRCh38): 5:141,318,490-141,320,784 (from NCBI)
Chiang and Roeder (1995) reported the cloning of a subunit of the TFIID protein complex (see 313650), which is required for transcription by promoters targeted by RNA polymerase II. The TFIID complex binds to the TATA box in class II promoters and then recruits other factors as well as RNA polymerase II. TFIID is composed of the TATA-binding protein (TBP; 600075) and multiple TBP-associated factors (TAFs), one of which has a predicted size of 55 kD from SDS gel electrophoresis. The human TFIID subunit TAF2F (also referred to as TAFII55) was isolated from a cell line that expresses an epitope-tagged TBP allowing for the immunoprecipitation of the TFIID complex and associated factors. Based on partial peptide sequence of 1 TAF, Chiang and Roeder (1995) designed degenerate PCR primers and used them to produce a probe which was, in turn, hybridized to a human placenta cDNA library. The predicted protein is 349 amino acids (40 kD) and contains 40% charged residues, which may account for its larger than expected electrophoretic mobility. The mRNA was expressed in all tissues examined. The authors showed that TAFII55 interacts with TAFII230, the largest subunit of TFIID, and with multiple transcription activators, including Sp1 (189906), YY1 (600013), USF (191523), CTF (600729; discussed also in 164005), and adenoviral E1A (discussed in 607102).
Gegonne et al. (2006) stated that TAF7 binds to TAF1 (313650) and inhibits its acetyltransferase activity, resulting in transcriptional repression. They found that TAF7 bound to TAF1 and associated with TFIID during formation of the preinitiation complex and dissociated from the preinitiation complex upon transcriptional initiation. Addition of polymerase II to the assembling preinitiation complex was associated with TAF1 and TAF7 phosphorylation and the release of TAF7. Gegonne et al. (2006) proposed that TAF7 is a checkpoint regulator suppressing premature transcription initiation until assembly of the preinitiation complex is complete.
By genomic sequence analysis, Wu et al. (2001) determined that the mouse and human TAF2F genes contain a single exon.
By genomic sequence analysis, Wu et al. (2001) mapped the TAF7 (TAF2F) gene to 5q31 on the opposite strand between the PCDHB (604967) and PCDHG (604968) gene clusters. They mapped the mouse Taf2f gene and PCDH gene clusters to chromosome 18c.
Chiang, C.-M., Roeder, R. G. Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators. Science 267: 531-536, 1995. [PubMed: 7824954] [Full Text: https://doi.org/10.1126/science.7824954]
Gegonne, A., Weissman, J. D., Zhou, M., Brady, J. N., Singer, D. S. TAF7: a possible transcription initiation check-point regulator. Proc. Nat. Acad. Sci. 103: 602-607, 2006. [PubMed: 16407123] [Full Text: https://doi.org/10.1073/pnas.0510031103]
Wu, Q., Zhang, T., Cheng, J.-F., Kim, Y., Grimwood, J., Schmutz, J., Dickson, M., Noonan, J. P., Zhang, M. Q., Myers, R. M., Maniatis, T. Comparative DNA sequence analysis of mouse and human protocadherin gene clusters. Genome Res. 11: 389-404, 2001. [PubMed: 11230163] [Full Text: https://doi.org/10.1101/gr.167301]