Alternative titles; symbols
HGNC Approved Gene Symbol: EPHB1
Cytogenetic location: 3q22.2 Genomic coordinates (GRCh38): 3:134,795,260-135,260,467 (from NCBI)
See 179610 for background on Eph receptors and their ligands, the ephrins.
Tang et al. (1995) cloned and characterized a member of the EPH-related receptor protein tyrosine kinases and designated it NET (neuronally expressed EPH-related tyrosine kinase). The cDNA was isolated from a fetal brain expression library using a monoclonal antibody. The 3.9-kb RNA encodes a predicted protein of 984 amino acids with 2 hydrophobic regions corresponding to possible signal peptide and transmembrane domains. The NET protein shares 99% amino acid identity with Elk, the rat homolog. Northern blots showed maximal NET expression in the nervous system and in some tumor cell lines derived from neuroectoderm.
Rissoan et al. (2002) detected expression of EPHB1 mRNA in several lymphoid cells. It was expressed at high levels in plasmacytoid dendritic cells and at much lower levels in monocytes, monocyte-derived dendritic cells, B cells, and CD34 (142230)-derived dendritic cells. Within plasmacytoid dendritic cells, immunostaining was clearly seen in the cytoplasm and in dendritic pseudopods following activation. Granular staining was revealed within the nucleus of monocyte-derived dendritic cells.
Using immunohistochemical analysis of the developing mouse hindbrain, Cowan et al. (2000) detected Ephb1 expression in the floor plate and in hindbrain regions where facial and inner ear efferent neurons are located.
Contractor et al. (2002) reported that mossy fiber long-term potentiation was reduced by perfusion of postsynaptic neurons with peptides and antibodies that interfere with binding of EphB receptor tyrosine kinases to the PDZ protein GRIP (GRIP1; 604597). Mossy fiber long-term potentiation was also reduced by extracellular application of soluble forms of beta-ephrins, which are normally membrane-anchored presynaptic ligands for the EphB receptors. The application of soluble ligands for presynaptic ephrins increased basal excitatory transmission and occluded both tetanus and forskolin-induced synaptic potentiation. Contractor et al. (2002) concluded that the PDZ interactions in postsynaptic neuron and transsynaptic interactions between postsynaptic EphB receptors and presynaptic beta-ephrins are necessary for the induction of mossy fiber long-term potentiation.
Batlle et al. (2005) showed that although Wnt (see 164820) signaling remains constitutively active, most human colorectal cancers lose expression of EphB at the adenoma-carcinoma transition. They found that loss of EphB expression strongly correlated with degree of malignancy. Furthermore, reduction of EphB activity accelerated tumorigenesis in the colon and rectum of Apc(Min/+) mice (see 611731), and resulted in formation of aggressive adenocarcinomas. Batlle et al. (2005) concluded that loss of EphB expression represents a critical step in colorectal cancer progression.
Tang et al. (1995) mapped the EPHB1 gene to 3q21-q23 by fluorescence in situ hybridization.
Batlle, E., Bacani, J., Begthel, H., Jonkeer, S., Gregorieff, A., van de Born, M., Malats, N., Sancho, E., Boon, E., Pawson, T., Gallinger, S., Pals, S., Clevers, H. EphB receptor activity suppresses colorectal cancer progression. Nature 435: 1126-1130, 2005. Note: Erratum: Nature 436: 881 only, 2005. [PubMed: 15973414] [Full Text: https://doi.org/10.1038/nature03626]
Contractor, A., Rogers, C., Maron, C., Henkemeyer, M., Swanson, G. T., Heinemann, S. F. Trans-synaptic Eph receptor-ephrin signaling in hippocampal mossy fiber LTP. Science 296: 1864-1869, 2002. [PubMed: 12052960] [Full Text: https://doi.org/10.1126/science.1069081]
Cowan, C. A., Yokoyama, N., Bianchi, L. M., Henkemeyer, M., Fritzsch, B. EphB2 guides axons at the midline and is necessary for normal vestibular function. Neuron 26: 417-430, 2000. [PubMed: 10839360] [Full Text: https://doi.org/10.1016/s0896-6273(00)81174-5]
Rissoan, M.-C., Duhen, T., Bridon, J.-M., Bendriss-Vermare, N., Peronne, C., Saint Vis, B., Briere, F., Bates, E. E. M. Subtractive hybridization reveals the expression of immunoglobulinlike transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood 100: 3295-3303, 2002. [PubMed: 12384430] [Full Text: https://doi.org/10.1182/blood-2002-02-0638]
Tang, X. X., Biegel, J. A., Nycum, L. M., Yoshioka, A., Brodeur, G. M., Pleasure, D. E., Ikegaki, N. cDNA cloning, molecular characterization, and chromosomal localization of NET (EPHT2), a human EPH-related receptor protein-tyrosine kinase gene preferentially expressed in brain. Genomics 29: 426-437, 1995. [PubMed: 8666391] [Full Text: https://doi.org/10.1006/geno.1995.9985]