Alternative titles; symbols
HGNC Approved Gene Symbol: NBL1
Cytogenetic location: 1p36.13 Genomic coordinates (GRCh38): 1:19,643,229-19,658,452 (from NCBI)
The NBL1 gene, also known as DAN (for 'differential screening-selected gene aberrant in neuroblastoma'), was originally cloned from a normal rat fibroblast cDNA library by a differential screening method (Ozaki and Sakiyama, 1993).
Enomoto et al. (1994) isolated the human DAN counterpart from a normal lung cDNA library by using rat DAN cDNA as a probe. They determined that the DAN gene encodes a transcription factor homologous to a mouse tumor suppressor gene.
By fluorescence in situ hybridization, Enomoto et al. (1994) mapped the human DAN gene to chromosome 1p36.13-p36.11.
By genetic mapping, the DAN gene is known to lie in 1p36, proximal to TNFR2 (191191) and distal to ID3 (600277) (White et al., 1995).
Matsuda et al. (1996) mapped the homologous gene to mouse chromosome 4 (band D3), and to rat 5q36.13. This is another example of homology between mouse chromosome 4 and human 1p.
Ozaki and Sakiyama (1994) found that the DAN gene possessed a tumor suppressive activity when overexpressed in v-src transformed cells.
By Southern blot analysis of tumor DNA from 26 patients with neuroblastoma (256000), Enomoto et al. (1994) found that 3 patients had genomic rearrangement or deletion of 1p36 within or closely linked to the DAN gene locus, suggesting that the DAN gene is a candidate tumor suppressor gene for human neuroblastoma. However, White et al. (1995) excluded DAN as a candidate gene for neuroblastoma through loss of heterozygosity (LOH) studies. The DAN gene was present in 2 copies in 3 neuroblastoma cell lines containing 1p deletions.
Enomoto, H., Ozaki, T., Takahashi, E., Nomura, N., Tabata, S., Takahashi, H., Ohnuma, N., Tanabe, M., Iwai, J., Yoshida, H., Matsunaga, T., Sakiyama, S. Identification of human DAN gene, mapping to the putative neuroblastoma tumor suppressor locus. Oncogene 9: 2785-2791, 1994. [PubMed: 8084583]
Matsuda, Y., Ozaki, T., Enomoto, H., Saito, T., Sakiyama, S. Chromosome mapping of the mouse and rat homologs of the human DAN gene, D1S1733E. Mammalian Genome 7: 709-710, 1996. [PubMed: 8703135] [Full Text: https://doi.org/10.1007/s003359900216]
Ozaki, T., Sakiyama, S. Tumor-suppressive activity of N03 gene product in v-src-transformed rat 3Y1 fibroblasts. Cancer Res. 54: 646-648, 1994. [PubMed: 8306325]
Ozaki, T., Sakiyama, S. Molecular cloning and characterization of a cDNA showing negative regulation in v-src-transformed 3Y1 rat fibroblasts. Proc. Nat. Acad. Sci. 90: 2593-2597, 1993. [PubMed: 8385338] [Full Text: https://doi.org/10.1073/pnas.90.7.2593]
White, P. S., Maris, J. M., Beltinger, C., Sulman, E., Marshall, H. N., Fujimori, M., Kaufman, B. A., Biegel, J. A., Allen, C., Hilliard, C., Valentine, M. B., Look, A. T., Enomoto, H., Sakiyama, S., Brodeur, G. M. A region of consistent deletion in neuroblastoma maps within human chromosome 1p36.2-36.3. Proc. Nat. Acad. Sci. 92: 5520-5524, 1995. [PubMed: 7777541] [Full Text: https://doi.org/10.1073/pnas.92.12.5520]