Alternative titles; symbols
HGNC Approved Gene Symbol: LGALS3BP
Cytogenetic location: 17q25.3 Genomic coordinates (GRCh38): 17:78,971,255-78,979,923 (from NCBI)
Using the monoclonal antibody SP-2, Iacobelli et al. (1986), Iacobelli et al. (1988), and Iacobelli et al. (1993) identified a 90-kD protein, designated serum protein 90K, that was found at elevated concentrations in the serum of patients with various types of breast, lung, colorectal, ovary, and endometrial cancer. Koths et al. (1993) cloned a cDNA encoding 90K, which they called MAC2BP, from a human monocytic cell line cDNA library using degenerate PCR primers based on the protein sequence. MAC2BP is a 585-amino acid protein containing an N-terminal region that is 45% identical to the extracellular, cysteine-rich domain of macrophage scavenger receptor (MSR1; 153622), indicating that MAC2BP is a member of the MSR1 superfamily. Koths et al. (1993) showed by immunoblotting that breast milk, serum, semen, saliva, urine, and tears likely contain MAC2B.
Ullrich et al. (1994) found by Northern blot analysis that all examined normal tissues, with the exception of peripheral blood lymphocytes, contain high levels of a 2.2-kb 90K transcript, with the highest levels present in the gastrointestinal tissues. They also showed that primary tumor samples and tumor cell lines express the 90K transcript at widely varying levels.
Koths et al. (1993) found that MAC2BP specifically bound the human macrophage-associated lectin LGALS3 (153619) via a carbohydrate-specific interaction.
Ullrich et al. (1994) noted that 90K is present at elevated levels in the serum of subpopulations of patients with AIDS. They demonstrated that 90K could stimulate host defense systems, such as natural killer cell and lymphokine-activated killer cell activities, and could induce secretion of interleukin-2 (IL2; 147680). Ullrich et al. (1994) suggested that oncogenic transformation, viral infection, or invasion by pathogens enhances the production of 90K, which then stimulates the host immune system via induction of IL2 and possibly other cytokines.
Using affinity chromatography and mass spectrometric analysis, Nonaka et al. (2011) identified MAC2BP as a ligand for the lectin receptor DCSIGN (CD209; 604672). DCSIGN recognized MAC2BP derived from some colorectal carcinomas, but not from others. Recognition depended on the presence of alpha-1,3,4-fucose moieties of Le glycans expressed on DCSIGN-binding MAC2BP.
Calabrese et al. (1995) mapped the human 90K gene to chromosome 17q25 by FISH. Brakebusch et al. (1997) used FISH to localize the mouse 90K gene to chromosome 11, region E.
Brakebusch, C., Jallal, B., Fusco, O., Iacobelli, S., Ullrich, A. Expression of the 90K immunostimulator gene is controlled by a promoter with unique features. J. Biol. Chem. 272: 3674-3682, 1997. [PubMed: 9013622] [Full Text: https://doi.org/10.1074/jbc.272.6.3674]
Calabrese, G., Sures, I., Pompetti, F., Natoli, G., Palka, G., Iacobelli, S. The gene (LGALS3BP) encoding the serum protein 90K, associated with cancer and infection by the human immunodeficiency virus, maps at 17q25. Cytogenet. Cell Genet. 69: 223-225, 1995. [PubMed: 7698018] [Full Text: https://doi.org/10.1159/000133969]
Iacobelli, S., Arno, E., D'Orazio, A., Coletti, G. Detection of antigens recognized by a novel monoclonal antibody in tissue and serum from patients with breast cancer. Cancer Res. 46: 3005-3010, 1986. [PubMed: 3516389]
Iacobelli, S., Arno, E., Sismondi, P., Natoli, C., Gentiloni, N., Scambia, G., Giai, M., Cortese, P., Panici, P. B., Mancuso, S. Measurement of a breast cancer associated antigen detected by monoclonal antibody (SP-2) in sera of cancer patients. Breast Cancer Res. Treat. 11: 19-30, 1988. [PubMed: 3164223] [Full Text: https://doi.org/10.1007/BF01807554]
Iacobelli, S., Bucci, I., D'Egidio, M., Giuliani, C., Natoli, C., Tinari, N., Rubistein, M., Schlessinger, J. Purification and characterization of a 90 kDa protein released from human tumors and tumor cell lines. FEBS Lett. 319: 59-65, 1993. [PubMed: 8454062] [Full Text: https://doi.org/10.1016/0014-5793(93)80037-u]
Koths, K., Taylor, E., Halenbeck, R., Casipit, C., Wang, A. Cloning and characterization of a human Mac-2-binding protein, a new member of the superfamily defined by the macrophage scavenger receptor cysteine-rich domain. J. Biol. Chem. 268: 14245-14249, 1993. [PubMed: 8390986]
Nonaka, M., Ma, B. Y., Imaeda, H., Kawabe, K., Kawasaki, N., Hodohara, K., Kawasaki, N., Andoh, A., Fujiyama, Y., Kawasaki, T. Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) recognizes a novel ligand, Mac-2-binding protein, characteristically expressed on human colorectal carcinomas. J. Biol. Chem. 286: 22403-22413, 2011. [PubMed: 21515679] [Full Text: https://doi.org/10.1074/jbc.M110.215301]
Ullrich, A., Sures, I., D'Egidio, M., Jallal, B., Powell, T. J., Herbst, R., Dreps, A., Azam, M., Rubinstein, M., Natoli, C., Shawver, L. K., Schlessinger, J., Iacobelli, S. The secreted tumor-associated antigen 90K is a potent immune stimulator. J. Biol. Chem. 269: 18401-18407, 1994. [PubMed: 8034587]