Alternative titles; symbols
HGNC Approved Gene Symbol: HNRNPF
Cytogenetic location: 10q11.21 Genomic coordinates (GRCh38): 10:43,385,618-43,409,186 (from NCBI)
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that bind heterogeneous nuclear RNA (hnRNA), the transcripts produced by RNA polymerase II (see 180660). More than 20 such proteins have been described and designated with letters from A to U. By molecular cDNA cloning, 2-dimensional gel immunoblotting, and amino acid microsequencing, Honore et al. (1995) identified 3 sequence-unique and distinct proteins that constitute a subfamily of ubiquitously expressed hnRNPs. The identity between hnRNPs H (601035) and H-prime (300610) was 96%, between H and F, 78%, and between H-prime and F, 75%.
Van Dusen et al. (2010) noted that human HNRNPH1 and HNRNPF have 3 RNA-binding domains and 2 glycine-rich domains and localize to both nucleus and cytoplasm. Using deletion mutants, they found that the central GYR (gly-tyr-arg) domain was required for nuclear localization. They identified a nonclassical nuclear localization signal within the GYR domain that interacted with transportin-1 (TNPO1; 602901), which mediated nuclear import. Van Dusen et al. (2010) concluded that HNRNPH1 and HNRNPF are transcription- and GYR domain-dependent shuttling proteins.
By fluorescence in situ hybridization, Honore et al. (1995) mapped the HNRNPF gene to 10q11.21-q11.22. McDonald et al. (1992) had previously mapped the HNRNPF gene to 10q11.2.
Honore, B., Rasmussen, H. H., Vorum, H., Dejgaard, K., Liu, X., Gromov, P., Madsen, P., Gesser, B., Tommerup, N., Celis, J. E. Heterogeneous nuclear ribonucleoproteins H, H-prime, and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes. J. Biol. Chem. 270: 28780-28789, 1995. [PubMed: 7499401] [Full Text: https://doi.org/10.1074/jbc.270.48.28780]
McDonald, H., Smailus, D., Jenkins, H., Adams, K., Simpson, N. E., Goodfellow, P. J. Identification and characterization of a gene at D10S94 in the MEN2A region. Genomics 13: 344-348, 1992. [PubMed: 1351868] [Full Text: https://doi.org/10.1016/0888-7543(92)90251-m]
Van Dusen, C. M., Yee, L., McNally, L. M., McNally, M. T. A glycine-rich domain of hnRNP H/F promotes nucleocytoplasmic shuttling and nuclear import through an interaction with transportin 1. Molec. Cell. Biol. 30: 2552-2562, 2010. [PubMed: 20308327] [Full Text: https://doi.org/10.1128/MCB.00230-09]