Alternative titles; symbols
HGNC Approved Gene Symbol: ADGRE5
Cytogenetic location: 19p13.12 Genomic coordinates (GRCh38): 19:14,381,444-14,408,723 (from NCBI)
CD97 is a 75- to 85-kD monomeric glycoprotein that is present on the surface of most activated leukocytes. Hamann et al. (1995) reported the isolation of a cDNA encoding human CD97 by expression cloning in COS cells. The cDNA encodes a 722-amino acid predicted protein whose C-terminal region contains 7 hydrophobic domains as seen in various G-protein associated transmembrane receptors. Sequence comparison indicated that CD97 is a member of a superfamily that includes the receptors for secretin (182099), calcitonin (114130), and other mammalian and insect peptide hormones. CD97 has an extracellular region of 433 amino acids with 3 N-terminal epidermal growth factor-like domains (2 of them with a calcium-binding site) and a single arg-gly-asp (RGD) motif. The EMR1 (600493) protein is similarly organized with N-terminal EGF domains and 7 transmembrane spanning regions and is about 31% identical to CD97.
Hamann et al. (1995) noted that the presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that CD97 is a receptor involved in both cell adhesion and signaling.
Hamann et al. (1996) found that CD55 (125240) is the cellular ligand for CD97.
Hamann et al. (1996) showed that the CD97 gene contains 18 exons spanning about 12 kb of genomic DNA. The authors speculated that CD97 arose by the shuffling of EGF-encoding exons at the 5-prime end of an ancestral member of the secretin receptor gene family.
Hamann et al. (1995) mapped the CD97 gene to 19p13.2-p13.12 by fluorescence in situ hybridization.
Hamann, J., Eichler, W., Hamann, D., Kerstens, H. M. J., Poddighe, P. J., Hoovers, J. M. N., Hartmann, E., Strauss, M., van Lier, R. A. W. Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain. J. Immun. 155: 1942-1950, 1995. [PubMed: 7636245]
Hamann, J., Hartmann, E., van Lier, R. A. W. Structure of the human CD97 gene: exon shuffling has generated a new type of seven-span transmembrane molecule related to the secretin receptor superfamily. Genomics 32: 144-147, 1996. [PubMed: 8786105] [Full Text: https://doi.org/10.1006/geno.1996.0092]
Hamann, J., Vogel, B., van Schijndel, G. M., van Lier, R. A. The seven-span transmembrane receptor CD97 has a cellular ligand (CD55, DAF). J. Exp. Med. 184: 1185-1189, 1996. [PubMed: 9064337] [Full Text: https://doi.org/10.1084/jem.184.3.1185]