Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF177
Cytogenetic location: 19p13.2 Genomic coordinates (GRCh38): 19:9,363,013-9,382,617 (from NCBI)
Baban et al. (1996) described 2 alternatively spliced cDNAs from a zinc finger gene designated ZNF177. The cDNAs were obtained from an NTera2D1 teratocarcinoma library and differ both at their 5-prime termini and by the presence or absence of a 559-bp internal exon. The longer cDNA (termed SB2) has a KRAB domain followed by 6 imperfect zinc finger motifs and 7 perfect C(2)H(2) motifs. However, a frameshift producing a terminator codon occurs after the KRAB domain. The shorter cDNA (termed SB1) encodes the KRAB domain and the C(2)H(2) type zinc fingers. Baban et al. (1996) found that each cDNA includes a sequence block containing a fragment of the env gene from HERV-H (Human Endogenous Retrovirus-H) and a partial Alu repeat. Both of the sequence blocks are located between unique 5-prime termini and the translation initiation site and both are inverted with respect to the 5-prime-to-3-prime gene orientation. Baban et al. (1996) showed that ZNF177 occurs at low levels in several cell types.
Landry et al. (2001) determined that the ZNF177 gene spans 58.7 kb and includes 9 exons, the last of which encodes the zinc finger motifs.
Landry et al. (2001) noted that the ZNF177 gene incorporates Alu, L1, and HERV segments into the 5-prime untranslated region (UTR) of transcripts. Landry et al. (2001) found that exon 4, present in all 3 alternative transcripts and thought to contain only Alu sequences (Baban et al., 1996), is actually composed of both Alu and L1 sequence. As is the case of the HERV element from which exon 2 is derived, both the Alu and L1 elements integrated in the opposite transcriptional direction relative to ZNF177. Using luciferase and GFP reporter constructs, Landry et al. (2001) demonstrated that the Alu and L1 sequences altered both the RNA and protein levels of reporter genes by increasing transcriptional efficiency while decreasing translational efficiency, suggesting that the Alu-L1 sequence in the 5-prime UTR of ZNF177 likely modulates gene expression by increasing transcription while impeding translation.
Using genomic sequence analysis, Landry et al. (2001) mapped the ZNF177 gene to chromosome 19p13.
Baban, S., Freeman, J. D., Mager, D. L. Transcripts from a novel human KRAB zinc finger gene contain spliced Alu and endogenous retroviral segments. Genomics 33: 463-472, 1996. [PubMed: 8661005] [Full Text: https://doi.org/10.1006/geno.1996.0221]
Landry, J.-R., Medstrand, P., Mager, D. L. Repetitive elements in the 5-prime untranslated region of a human zinc-finger gene modulate transcription and translation efficiency. Genomics 76: 110-116, 2001. [PubMed: 11549323] [Full Text: https://doi.org/10.1006/geno.2001.6604]