Alternative titles; symbols
HGNC Approved Gene Symbol: PSMC5
Cytogenetic location: 17q23.3 Genomic coordinates (GRCh38): 17:63,827,431-63,832,019 (from NCBI)
PSMC5 is a member of the AAA (ATPases Associated with diverse cellular Activities) gene family (Hoyle et al., 1997).
Members of the AAA gene family are most similar throughout an approximately 230-amino acid ATPase domain (the AAA domain), and related genes have been described in organisms as distant as archaebacteria and eubacteria. All the known AAA proteins contain either 1 or 2 AAA domains, and the family can be subdivided into 2 groups on this basis. It appears that 2-domain AAA proteins (e.g., NSF, 601633) are associated with membrane functions. In eukaryotes, the 1-domain AAA proteins such as PSMC5 appear to be involved largely in the regulatory subunit of the 26S protease (summary by Hoyle et al., 1997).
Lee et al. (1995) used the yeast interaction trap, a version of the yeast 2-hybrid system, to identify proteins that specifically interact with the ligand-binding domain of rat thyroid hormone receptor-beta (THRB; 190160). They isolated HeLa cell cDNAs encoding several different thyroid hormone receptor (TR)-interacting proteins (TRIPs), including TRIP1 (PSMC5). TRIP1 strongly interacted with rat Thrb only in the presence of thyroid hormone. It also showed a ligand-dependent interaction with retinoid X receptor-alpha (RXRA; 180245), but did not interact with the glucocorticoid receptor (NR3C1; 138040) under any condition. The deduced 406-amino acid TRIP1 protein shares a high degree of sequence similarity with the yeast transcriptional coactivator SUG1. TRIP1 contains an AAA domain that is positioned toward the C terminus, which is typical in the AAA family. Lee et al. (1995) found that TRIP1 is ubiquitously expressed as an approximately 1.4-kb transcript.
Lee et al. (1995) showed that TRIP1 can functionally substitute for Sug1 in yeast, and that both proteins interact in vitro with the TR and with the transcriptional activation domains of yeast GAL4 and herpes virus VP16.
By PCR amplification of a partial sequence of PSMC5 in a panel of human monochromosomal cell hybrids, Hoyle et al. (1997) demonstrated that the PSMC5 gene is located on chromosome 17. By fluorescence in situ hybridization (FISH), they refined the localization to chromosome 17q24-q25. Tanahashi et al. (1998) mapped the PSMC5 gene to chromosome 17q23.1-q23.3 by FISH.
Hoyle, J., Tan, K. H., Fisher, E. M. C. Localization of genes encoding two human one-domain members of the AAA family: PSMC5 (the thyroid hormone receptor-interacting protein, TRIP1) and PSMC3 (the Tat-binding protein, TBP1). Hum. Genet. 99: 285-288, 1997. [PubMed: 9048938] [Full Text: https://doi.org/10.1007/s004390050356]
Lee, J. W., Choi, H.-S., Gyuris, J., Brent, R., Moore, D. D. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Molec. Endocr. 9: 243-254, 1995. [PubMed: 7776974] [Full Text: https://doi.org/10.1210/mend.9.2.7776974]
Lee, J. W., Ryan, F., Swaffield, J. C., Johnston, S. A., Moore, D. D. Interaction of thyroid-hormone receptor with a conserved transcriptional mediator. Nature 374: 91-94, 1995. [PubMed: 7870181] [Full Text: https://doi.org/10.1038/374091a0]
Tanahashi, N., Suzuki, M., Fujiwara, T., Takahashi, E., Shimbara, N., Chung, C. H., Tanaka, K. Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPases. Biochem. Biophys. Res. Commun. 243: 229-232, 1998. [PubMed: 9473509] [Full Text: https://doi.org/10.1006/bbrc.1997.7892]