Alternative titles; symbols
HGNC Approved Gene Symbol: NPY6R
Cytogenetic location: 5q31.2 Genomic coordinates (GRCh38): 5:137,801,193-137,810,751 (from NCBI)
Matsumoto et al. (1996) probed cDNA libraries with the neuropeptide Y (NPY; 162640) Y1 receptor gene (NPY1R; 162641) and cloned a novel NPY receptor, which they named Y2b, from several species, including rabbits, humans, and other primates. They noted that the Y2b receptor is the ortholog of the mouse Y5 receptor. While the rabbit Y2b receptor encodes a functional 371-amino acid protein, the human ortholog contains a premature stop codon, resulting in a 290-amino acid protein which has no receptor function. All primates tested had a truncated Y2b gene product, some having a second premature stop codon, thus encoding an even shorter polypeptide than the human. Northern blot analysis revealed that the Y2b gene is abundantly expressed in human heart and skeletal muscle as a 3-kb transcript. Matsumoto et al. (1996) suggested that Y2b may have acquired a novel function in primates.
Gregor et al. (1996) used degenerate PCR with primers based on known NPY receptors to clone a novel receptor, which they termed PP2, from human brain mRNA. They then cloned full-length cDNAs from a human heart cDNA library and also isolated a mouse genomic clone containing the entire coding region of the ortholog. Sequence analysis of the human PP2 gene indicated that it encodes a 290-amino acid polypeptide with one difference when compared to the mouse ortholog and to other NPY receptors: all human PP2 clones examined contained a single basepair deletion relative to the mouse ortholog, resulting in a truncated protein lacking the seventh transmembrane domain. Gregor et al. (1996) found that human PP2, when expressed, was unable to bind PP, NPY, or PYY, and these authors suggested that in humans, PP2 is either functionally inactive or has a PP-independent function.
Rose et al. (1997) isolated a Y1-like receptor clone from a human hypothalamus cDNA library. This clone was found to harbor a truncated, nonfunctional receptor sequence; to test the hypothesis that a mutant receptor clone may have been isolated, Rose et al. (1997) analyzed genomic DNA from 192 human subjects varying in age, ethnic background, and degree of obesity. The same truncated receptor sequence was found in all subjects, suggesting to Rose et al. (1997) that NPY6R may exist as a transcribed pseudogene in the human genome.
Rose et al. (1997) mapped the NPY6R gene to 5q31 by fluorescence in situ hybridization.
Lutz et al. (1997) mapped the Npy6r gene to mouse chromosome 18, approximately 21 cM from the centromere, where it is a member of a large conserved linkage group on human 5q21-q35.
Gregor, P., Feng, Y., DeCarr, L. B., Cornfield, L. J., McCaleb, M. L. Molecular characterization of a second mouse pancreatic polypeptide receptor and its inactivated human homologue. J. Biol. Chem. 271: 27776-27781, 1996. [PubMed: 8910373] [Full Text: https://doi.org/10.1074/jbc.271.44.27776]
Lutz, C. M., Richards, J. E., Scott, K. L., Sinha, S., Yang-Feng, T. L., Frankel, W. N., Thompson, D. A. Neuropeptide Y receptor genes mapped in human and mouse: receptors with high affinity for pancreatic polypeptide are not clustered with receptors specific for neuropeptide Y and peptide YY. Genomics 46: 287-290, 1997. [PubMed: 9417917] [Full Text: https://doi.org/10.1006/geno.1997.5024]
Matsumoto, M., Nomura, T., Momose, K., Ikeda, Y., Kondou, Y., Akiho, H., Togami, J., Kimura, Y., Okada, M., Yamaguchi, T. Inactivation of a novel neuropeptide Y/peptide YY receptor gene in primate species. J. Biol. Chem. 271: 27217-27220, 1996. [PubMed: 8910290] [Full Text: https://doi.org/10.1074/jbc.271.44.27217]
Rose, P. M., Lynch, J. S., Frazier, S. T., Fisher, S. M., Chung, W., Battaglino, P., Fathi, Z., Leibel, R., Fernandes, P. Molecular genetic analysis of a human neuropeptide Y receptor. J. Biol. Chem. 272: 3622-3627, 1997. [PubMed: 9013614] [Full Text: https://doi.org/10.1074/jbc.272.6.3622]