Alternative titles; symbols
HGNC Approved Gene Symbol: PTK6
Cytogenetic location: 20q13.33 Genomic coordinates (GRCh38): 20:63,528,001-63,537,376 (from NCBI)
PTK6 is a nonreceptor protein tyrosine kinase (Kang et al., 2002).
Mitchell et al. (1994) used a PCR-based differential screening procedure to isolate a novel protein-tyrosine kinase that they termed BRK for 'breast tumor kinase.' They found that the full-length cDNA, cloned from human breast tumor cell lines, was similar to other tumor-related kinases, particularly those of the SRC family (e.g., 190090 and 124095). The deduced 451-amino acid polypeptide sequence was composed of 3 domains: an SH3 domain, an SH2 domain, and a catalytic domain. The sequence of BRK, unlike that of SRC, does not include an N-terminal myristoylation domain. Using Northern blotting and RT-PCR, Mitchell et al. (1994) detected low levels of BRK transcripts in some breast tumor cell lines, but not in normal breast tissue or other tissues tested.
Mitchell et al. (1997) identified a splice variant of PTK6 that encodes a 134-amino acid protein with a calculated molecular mass of 15 kD. This variant contains the N-terminal SH3 domain found in the longer isoform, followed by a novel proline-rich sequence. Western blot analysis detected both isoforms in a breast cancer cell line.
Lee et al. (1998) found that PTK6 mRNA is expressed at very high levels in colon, at high levels in small intestine and prostate, and at low levels in some fetal tissues.
Mitchell et al. (1994) showed that BRK is capable of tyrosine autophosphorylation. Kamalati et al. (1996) found that overexpression of BRK in mammary epithelial cells led to sensitization of cells to epidermal growth factor (EGF; 131530) and resulted in a partially transformed phenotype. They also demonstrated coimmunoprecipitation of BRK and the EGF receptor (131550). Mutational analysis suggested to Kamalati et al. (1996) that while SRC and BRK share some functional properties, they function differently during transformation.
Mitchell et al. (1997) determined that the PTK6 gene contains 8 exons and spans 10 kb. Lee et al. (1998) determined that the PTK6 gene contains 8 exons and spans approximately 8.8 kb. Kang et al. (2002) analyzed the promoter region of the PTK6 gene by gel mobility shift assays and identified interaction with NF-kappa-B (see 164011) and SP1 (189906).
By FISH, Park et al. (1997) mapped the PTK6 gene to chromosome 20q13.3.
Kamalati, T., Jolin, H. E., Mitchell, P. J., Barker, K. T., Jackson, L. E., Dean, C. J., Page, M. J., Gusterson, B. A., Crompton, M. R. Brk, a breast tumor-derived non-receptor protein-tyrosine kinase, sensitizes mammary epithelial cells to epidermal growth factor. J. Biol. Chem. 271: 30956-30963, 1996. [PubMed: 8940083] [Full Text: https://doi.org/10.1074/jbc.271.48.30956]
Kang, K.-N., Kim, M., Pae, K.-M., Lee, S.-T. Characterization of the 5-prime-flanking region of the human PTK6 gene. Biochim. Biophys. Acta 1574: 365-369, 2002. [PubMed: 11997104] [Full Text: https://doi.org/10.1016/s0167-4781(02)00234-8]
Lee, H., Kim, M., Lee, K.-H., Kang, K.-N., Lee, S.-T. Exon-intron structure of the human PTK6 gene demonstrates that PTK6 constitutes a distinct family of non-receptor tyrosine kinase. Molec. Cells 8: 401-407, 1998. [PubMed: 9749526]
Mitchell, P. J., Barker, K. T., Martindale, J. E., Kamalati, T., Lowe, P. N., Page, M. J., Gusterson, B. A., Crompton, M. R. Cloning and characterisation of cDNAs encoding a novel non-receptor tyrosine kinase, brk, expressed in human breast tumours. Oncogene 9: 2383-2390, 1994. [PubMed: 8036022]
Mitchell, P. J., Barker, K. T., Shipley, J., Crompton, M. R. Characterisation and chromosome mapping of the human non receptor tyrosine kinase gene, brk. Oncogene 15: 1497-1502, 1997. Note: Erratum: Oncogene 17: 129 only, 1998. [PubMed: 9333026] [Full Text: https://doi.org/10.1038/sj.onc.1201292]
Park, S.-H., Lee, K.-H., Kim, H., Lee, S.-T. Assignment of the human PTK6 gene encoding a non-receptor protein tyrosine kinase to 20q13.3 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 77: 271-272, 1997. [PubMed: 9284935] [Full Text: https://doi.org/10.1159/000134595]