Alternative titles; symbols
HGNC Approved Gene Symbol: ZBTB14
Cytogenetic location: 18p11.31 Genomic coordinates (GRCh38): 18:5,289,022-5,297,053 (from NCBI)
Sugiura et al. (1997) used antisera from patients with autoimmune disorders to isolate novel genes by expression screening. Using the antisera of a 41-year-old woman diagnosed with dermatomyositis and scleroderma, they isolated the human homolog of mouse gene ZF5 from a HeLa cell cDNA library. The gene encodes a zinc finger protein of 449 amino acids with a predicted mass of 51 kD. The deduced amino acid sequence is 99% identical to mouse ZF5 and 95% identical to chicken ZF5. Mouse ZF5 has been shown to be a transcription repressor of c-myc (190080) and tk (188300) promoters. The ZF5 sequence contains a hydrophobic domain, known as BTB/POZ, near its N terminus. Sugiura et al. (1997) used immunofluorescence to localize the ZF5 protein in transfected COS-7 cells. Full-length ZF5 protein was located in discrete dots in the nucleus, while ZF5 lacking the BTB/POZ domain was located in the nuclear periphery, suggesting to the authors that the BTB/POZ domain directs the protein to specific sites in the nucleus.
Based on its ability to bind to a zinc chelate column, Sobek-Klocke et al. (1997) cloned ZFP161 from a HeLa cell cDNA expression library. They found that the protein contains 5 Kruppel-type C2H2 zinc fingers near its C terminus.
Using somatic cell hybrids, Sobek-Klocke et al. (1997) mapped the ZFP161 gene to human chromosome 18pter-p11.21. The authors mapped the mouse Zfp161 homolog to mouse chromosome 17, but noted that there was a closely-related gene on the mouse X chromosome.
Sobek-Klocke et al. (1997) proposed ZFP161 as a candidate gene for holoprosencephaly type 4 (HPE4; 142946).
Sobek-Klocke, I., Disque-Kochem, C., Ronsiek, M., Klocke, R., Jockusch, H., Breuning, A., Ponstingl, H., Rojas, K., Overhauser, J., Eichenlaub-Ritter, U. The human gene ZFP161 on 18p11.21-pter encodes a putative c-myc repressor and is homologous to murine Zfp161 (chr 17) and Zfp161-rs1 (X chr). Genomics 43: 156-164, 1997. Note: Erratum: Genomics 45: 633 only, 1997. [PubMed: 9244432] [Full Text: https://doi.org/10.1006/geno.1997.4784]
Sugiura, K., Muro, Y., Nagai, Y., Kamimoto, T., Wakabayashi, T., Ohashi, M., Hagiwara, M. Expression cloning and intracellular localization of a human ZF5 homologue. Biochim. Biophys. Acta 1352: 23-26, 1997. [PubMed: 9177479] [Full Text: https://doi.org/10.1016/s0167-4781(97)00045-6]