Alternative titles; symbols
HGNC Approved Gene Symbol: CCL8
Cytogenetic location: 17q12 Genomic coordinates (GRCh38): 17:34,319,435-34,321,402 (from NCBI)
Chemokines, a family of small cytokines, attract leukocytes and possess various immunomodulating functions. The C-C chemokines influence mononuclear cell types. Van Damme et al. (1992) isolated MCP2 from stimulated osteosarcoma cells, determined its nearly complete amino acid sequence, and identified it as a monocyte chemotactic factor based on a pattern of conserved cysteine residues. The mature MCP2 protein is approximately 75 amino acids long Van Damme et al. (1992).
Van Coillie et al. (1997) stated that the MCP2 gene encodes a predicted 109-amino acid protein. They reported that the predicted protein sequence matches the sequence of purified mature MCP2 reported by Van Damme et al. (1992) with the exception of 1 lysine-to-glutamine change, which Van Coillie et al. (1997) considered a polymorphism or cloning artifact. By Northern blot analysis, Van Coillie et al. (1997) detected a major 1-kb transcript in most tissues tested, with greatest expression in small intestine and peripheral blood cells. Transcripts of 1.5 and 2.4 kb were also observed.
Van Coillie et al. (1997) stated that the MCP2 gene contains 3 exons in a span of about 3 kb. Van Coillie et al. (1997) deduced that intron/exon structure was conserved among MCP1 (158105), MCP2, and MCP3 (158106), and reported that these genes share 77% coding nucleotide sequence homology.
By degenerate PCR with primers based on C-C chemokine consensus sequences, Van Coillie et al. (1997) isolated the MCP2 gene from a YAC contig from human chromosome 17q11.2.
Van Coillie, E., Fiten, P., Nomiyama, H., Sakaki, Y., Miura, R., Yoshie, O., Van Damme, J., Opdenakker, G. The human MCP-2 gene (SCYA8): cloning, sequence analysis, tissue expression, and assignment to the CC chemokine gene contig on chromosome 17q11.2. Genomics 40: 323-331, 1997. [PubMed: 9119400] [Full Text: https://doi.org/10.1006/geno.1996.4594]
Van Damme, J., Proost, P., Lenaerts, J.-P., Opdenakker, G. Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family. J. Exp. Med. 176: 59-65, 1992. [PubMed: 1613466] [Full Text: https://doi.org/10.1084/jem.176.1.59]