Alternative titles; symbols
HGNC Approved Gene Symbol: HNRNPH3
Cytogenetic location: 10q21.3 Genomic coordinates (GRCh38): 10:68,331,693-68,343,196 (from NCBI)
The RNAs found in the nucleus include pre-mRNAs, mature mRNAs, and intermediate reaction products. Collectively these RNAs are termed heterogeneous nuclear RNAs (hnRNAs). Ribonucleoprotein (RNP) complexes consisting of hnRNAs and associated proteins are responsible for RNA splicing and processing. Mahe et al. (1997) used antibodies against ribonucleoprotein complexes to clone the gene of 1 such protein, which the authors termed 2H9, from a human HeLa cell cDNA library. The sequence of 2H9 predicts a 346-amino acid polypeptide containing 2 putative RNA binding domains. The 2H9 gene shares significant homology with other RNPs, including HNRPH1 (601035), HNRPH2 (300610), and HNRPF (601037). Specific antibodies against 2H9 can inhibit in vitro splicing, suggesting that 2H9 is involved in this process. Immunofluorescence staining revealed that 2H9 is localized to the nucleus, particularly in nuclear bodies.
Mahe et al. (1997) used isotopic in situ hybridization to map the 2H9 gene to human chromosome 10q22.
Mahe, D., Mahl, P., Gattoni, R., Fischer, N., Mattei, M.-G., Stevenin, J., Fuchs, J.-P. Cloning of human 2H9 heterogeneous nuclear ribonucleoproteins. J. Biol. Chem. 272: 1827-1836, 1997. [PubMed: 8999868] [Full Text: https://doi.org/10.1074/jbc.272.3.1827]