Alternative titles; symbols
HGNC Approved Gene Symbol: SECTM1
Cytogenetic location: 17q25.3 Genomic coordinates (GRCh38): 17:82,321,024-82,333,959 (from NCBI)
While analyzing a DNase-hypersensitive region approximately 5 kb upstream of the CD7 (186820) gene at 17q25.2-q25.3, Slentz-Kesler et al. (1998) cloned a SECTM1 cDNA, which they called K12. Northern blot analysis revealed that K12 was expressed as a 1.8-kb mRNA in many of the tissues tested, with the highest level of expression in peripheral blood leukocytes. The predicted 248-amino acid K12 protein has the hydropathic characteristics of a type 1a transmembrane protein, which is defined by an extracellular N terminus and a cleaved signal peptide. The authors demonstrated that the K12 protein behaves biochemically as an integral membrane protein. Using immunofluorescence, they localized K12 to the Golgi. Western blotting showed that the K12 protein migrates as a cluster of bands at 27 kD. In addition, an approximately 20-kD soluble form of K12 is secreted by cells.
Slentz-Kesler et al. (1998) determined that the K12 gene contains 4 exons and spans approximately 14 kb.
Slentz-Kesler et al. (1998) identified the K12 gene on chromosome 17q25.2-q25.4.
Slentz-Kesler, K. A., Hale, L. P., Kaufman, R. E. Identification and characterization of K12 (SECTM1), a novel human gene that encodes a golgi-associated protein with transmembrane and secreted isoforms. Genomics 47: 327-340, 1998. [PubMed: 9480746] [Full Text: https://doi.org/10.1006/geno.1997.5151]