Alternative titles; symbols
HGNC Approved Gene Symbol: PPP1R8
Cytogenetic location: 1p35.3 Genomic coordinates (GRCh38): 1:27,830,782-27,851,676 (from NCBI)
In Escherichia coli, the rne gene encodes RNase E, a protein involved in RNA degradation. Wang and Cohen (1994) cloned a human gene, which they called ARD1 for 'activator of RNA decay,' that is able to complement mutations in the E. coli rne gene. The human ARD1 gene encodes a proline-rich 127-amino acid protein with a predicted mass of 13.3 kD. Wang and Cohen (1994) showed that human ARD1 protein is able to produce RNase E-specific cleavages in E. coli.
Van Eynde et al. (1995) cloned the bovine NIPP1 gene. The 351-amino acid NIPP1 protein is a specific inhibitor of type 1 serine/threonine protein phosphatases. The human ARD1 amino acid sequence is virtually identical to the carboxy terminus of the bovine NIPP1 amino acid sequence. Because the homology also extends into noncoding regions, the authors asserted that the NIPP1 and ARD1 proteins are alternately spliced products of the same gene.
Claverie-Martin et al. (1997) purified human ARD1 protein and found that its apparent size was 19 kD. They attributed the observed retarded mobility on SDS-PAGE to the highly charged residues at the ends of the protein. Enzyme assays showed that ARD1, like RNase E, is Mg2+ dependent and that ARD1 and RNase E cleave RNA at the same sites in A+U-rich regions.
Wang (1995) showed that the common 3-prime untranslated region of ARD1 and NIPP1 maps to human chromosome 1.
Claverie-Martin, F., Wang, M., Cohen, S. N. ARD-1 cDNA from human cells encodes a site-specific single-strand endoribonuclease that functionally resembles Escherichia coli RNase E. J. Biol. Chem. 272: 13823-13828, 1997. [PubMed: 9153239] [Full Text: https://doi.org/10.1074/jbc.272.21.13823]
Van Eynde, A., Wera, S., Beullens, M., Torrekens, S., Van Leuven, F., Stalmans, W., Bollen, M. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing. J. Biol. Chem. 270: 28068-28074, 1995. [PubMed: 7499293] [Full Text: https://doi.org/10.1074/jbc.270.47.28068]
Wang, M., Cohen, S. N. ard-1: a human gene that reverses the effects of temperature-sensitive and deletion mutations in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages. Proc. Nat. Acad. Sci. 91: 10591-10595, 1994. [PubMed: 7524097] [Full Text: https://doi.org/10.1073/pnas.91.22.10591]
Wang, M. Isolation and characterization of a human gene encoding a single-strand-specific endoribonuclease. Ph.D. Thesis: Stanford Univ. 1995.