Alternative titles; symbols
HGNC Approved Gene Symbol: UBE2K
Cytogenetic location: 4p14 Genomic coordinates (GRCh38): 4:39,698,136-39,782,792 (from NCBI)
The HIP2 gene encodes a ubiquitin-conjugating enzyme that interacts with huntingtin (HTT; 613004) (Kalchman et al., 1996).
By use of the yeast 2-hybrid system, Kalchman et al. (1996) identified the HIP2 protein. The 200-amino acid HIP2 protein sequence was identical to that of bovine ubiquitin-conjugating enzyme E2-25K. Assays of the interaction of HIP2 with huntingtin revealed that HIP2 binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. Northern blot analysis revealed that HIP2 was transcribed as 1.2- and 1.4-kb mRNAs in all human tissues studied. Western blot analysis detected protein bands of 25 kD, 28 kD, and 45 kD; the 28-kD band appeared to be selective for tissues of the central nervous system. Kalchman et al. (1996) showed that huntingtin is ubiquitinated in peripheral cells, suggesting a role for HIP2 in the degradation of huntingtin.
By fluorescence in situ hybridization, Kalchman et al. (1996) mapped the HIP2 gene to human chromosome 4p14.
Kalchman, M. A., Graham, R. K., Xia, G., Koide, H. B., Hodgson, J. G., Graham, K. C., Goldberg, Y. P., Gietz, R. D., Pickart, C. M., Hayden, M. R. Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J. Biol. Chem. 271: 19385-19394, 1996. [PubMed: 8702625] [Full Text: https://doi.org/10.1074/jbc.271.32.19385]