Alternative titles; symbols
HGNC Approved Gene Symbol: PTPRR
Cytogenetic location: 12q15 Genomic coordinates (GRCh38): 12:70,638,073-70,920,738 (from NCBI)
For general information about protein-tyrosine phosphatases (PTPs), see PTPRA (176884).
To clone cDNAs that encode PTPs expressed in the nervous system, Shiozuka et al. (1995) performed RT-PCR on rat PC12h cell mRNA, using degenerate oligonucleotides based on a conserved PTP domain. They isolated a full-length rat PTP cDNA, which they named Pcptp1, by screening a PC12h cell cDNA library with the PCR product. The longest open reading frame contains 6 methionine codons that can potentially serve as translational start sites. Western blot analysis of PC12h cell lysates using antibodies against the C-terminal region of Pcptp1 detected major 65-kD and minor 42-kD proteins, which correspond with translation beginning at the second and fifth methionine codons, respectively. Pcptp1 is a receptor-type PTP with a single PTP catalytic domain, which is located in the C-terminal cytoplasmic region. The amino acid sequence of the 42-kD form is 54% identical to that of the rat nonreceptor-type PTP Step (see PTPN5; 176879). Northern blot analysis of rat tissues detected a 3.9-kb Pcptp1 transcript in most parts of the brain and in adrenal gland, a 3.3-kb transcript almost exclusively in the cerebellum, and a 1.7-kb transcript in brain and many nonneuronal tissues. The authors deposited the nucleotide sequence of a human brain PCPTP1 cDNA in GenBank (D64053); the cDNA encodes 657 amino acids.
Gross (2014) mapped the PTPRR gene to chromosome 12q15 based on an alignment of the PTPRR sequence (GenBank BC110900) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 7/18/2014.
Shiozuka, K., Watanabe, Y., Ikeda, T., Hashimoto, S., Kawashima, H. Cloning and expression of PCPTP1 encoding protein tyrosine phosphatase. Gene 162: 279-284, 1995. [PubMed: 7557444] [Full Text: https://doi.org/10.1016/0378-1119(95)00306-q]