HGNC Approved Gene Symbol: LECT2
Cytogenetic location: 5q31.1 Genomic coordinates (GRCh38): 5:135,946,904-135,954,983 (from NCBI)
Neutrophils migrate in response to increasing concentrations of chemotactic factors released from inflammatory sites. From the culture fluid of a human T-cell leukemia cell line (SKW-3), Yamagoe et al. (1996) isolated a 16-kD secreted protein with neutrophil chemotactic activity and designated it LECT2. They purified the protein and determined the amino acid sequence. By carrying out RT-PCR with degenerate oligonucleotide primers based on the amino acid sequence, Yamagoe et al. (1998) cloned an SKW-3 cell cDNA encoding LECT2. The predicted 151-amino acid protein contained an 18-residue signal sequence. However, the sequence of the predicted mature protein was only 86% identical to that of purified LECT2. By cloning of the bovine homolog, Yamagoe et al. (1998) determined that the LECT2 protein purified by Yamagoe et al. (1996) was derived from fetal bovine serum in the SKW-3 cell culture medium. Using Northern blot analysis, Yamagoe et al. (1998) found that LECT2 is expressed as 1.3- and 1.0-kb mRNAs in adult and fetal liver. Low levels of the 1.0-kb transcript were observed in testis. The authors found that there is genetic polymorphism in the LECT2 gene.
Using immunofluorescence, Yamagoe et al. (1997) demonstrated that LECT2 is found in the cytoplasm of human hepatocytes and is also localized in some endothelial cells of hepatic arteries, portal veins, and central veins.
Yamagoe et al. (1998) stated that LECT2 is the same as bovine chondromodulin II, a positive regulator of chondrocyte proliferation, and is highly related to chicken mim1, a myeloblastosis-induced myeloid protein (see 189990) and CEBP-beta (189965) target gene.
Yamagoe et al. (1998) reported that the LECT2 gene contains 4 exons and spans approximately 8 kb. Primer extension assays revealed multiple initiation sites.
By analysis of a somatic cell hybrid panel and by fluorescence in situ hybridization, Yamagoe et al. (1998) mapped the LECT2 gene to 5q31.1-q32. By interspecific backcross analysis, Yamagoe et al. (1998) mapped the mouse Lect2 gene to chromosome 13.
Yamagoe, S., Akasaka, T., Uchida, T., Hachiya, T., Okabe, T., Yamakawa, Y., Arai, T., Mizuno, S., Suzuki, K. Expression of a neutrophil chemotactic protein LECT2 in human hepatocytes revealed by immunochemical studies using polyclonal and monoclonal antibodies to a recombinant LECT2. Biochem. Biophys. Res. Commun. 237: 116-120, 1997. [PubMed: 9266841] [Full Text: https://doi.org/10.1006/bbrc.1997.7095]
Yamagoe, S., Kameoka, Y., Hashimoto, K., Mizuno, S., Suzuki, K. Molecular cloning, structural characterization, and chromosomal mapping of the human LECT2 gene. Genomics 48: 324-329, 1998. [PubMed: 9545637] [Full Text: https://doi.org/10.1006/geno.1997.5198]
Yamagoe, S., Mizuno, S., Suzuki, K. Molecular cloning of human and bovine LECT2 having a neutrophil chemotactic activity and its specific expression in the liver. Biochim. Biophys. Acta 1396: 105-113, 1998. [PubMed: 9524238] [Full Text: https://doi.org/10.1016/s0167-4781(97)00181-4]
Yamagoe, S., Watanabe, T., Mizuno, S., Suzuki, K. The mouse Lect2 gene: cloning of cDNA and genomic DNA, structural characterization and chromosomal localization. Gene 216: 171-178, 1998. [PubMed: 9714793] [Full Text: https://doi.org/10.1016/s0378-1119(98)00294-7]
Yamagoe, S., Yamakawa, Y., Matsuo, Y., Minowada, J., Mizuno, S., Suzuki, K. Purification and primary amino acid sequence of a novel neutrophil chemotactic factor LECT2. Immun. Lett. 52: 9-13, 1996. [PubMed: 8877413] [Full Text: https://doi.org/10.1016/0165-2478(96)02572-2]