HGNC Approved Gene Symbol: SDF2
Cytogenetic location: 17q11.2 Genomic coordinates (GRCh38): 17:28,648,346-28,662,194 (from NCBI)
By use of the signal sequence trap method, Hamada et al. (1996) isolated a cDNA encoding a novel secretory protein, Sdf2, from the mouse stromal cell line ST2. The Sdf2 cDNA encodes a putative 211-amino acid protein that includes a 30-amino acid hydrophobic signal peptide. Because the protein lacks a hydrophobic region in addition to that at the N terminus, the authors stated that the protein is likely to be a secretory and not a membrane protein. Sdf2 has significant similarity to hydrophilic segments of yeast mannosyltransferases Pmt1p and Pmt2p which reside in the endoplasmic membrane and transfer mannose residues from dolichyl phosphate D-mannose to specific ser/thr residues. Northern blot analysis of adult mouse organ mRNA demonstrated ubiquitous expression of Sdf2. Southern blot analysis indicated that the SDF2 gene is relatively conserved among mammals. By use of the full-length Sdf2 mouse probe, Hamada et al. (1996) isolated the human homolog from a glioblastoma cell line. They found that the putative human SDF2 protein is 92.4% identical to that of the mouse.
By fluorescence in situ hybridization, Hamada et al. (1996) localized the human SDF2 gene to chromosome 17q11.2.
Hamada, T., Tashiro, K., Tada, H., Inazawa, J., Shirozu, M., Shibahara, K., Nakamura, T., Martina, N., Nakano, T., Honjo, T. Isolation and characterization of a novel secretory protein, stromal cell-derived factor-2 (SDF-2) using the signal sequence trap method. Gene 176: 211-214, 1996. [PubMed: 8918255] [Full Text: https://doi.org/10.1016/0378-1119(96)00251-x]