Alternative titles; symbols
HGNC Approved Gene Symbol: ENTPD3
Cytogenetic location: 3p22.1 Genomic coordinates (GRCh38): 3:40,387,184-40,428,744 (from NCBI)
Plasma membrane-bound ectonucleoside trisphosphate diphosphohydrolases (ENTPDases), such as ENTPD3, modulate P2 receptor (see 600845) signaling by controlling extracellular nucleotide concentrations via nucleoside tri- and diphosphate hydrolysis (Munkonda et al., 2007).
The catabolism of extracellular nucleotides is mediated by several types of ectonucleotidases, including divalent cation-dependent NTPases, such as CD39 (601752). By searching an EST database for sequences related to that of CD39, Chadwick and Frischauf (1998) identified CD39L3 cDNAs. The predicted 529-amino acid CD39L3 protein contains all 4 apyrase-conserved regions (ACRs) characteristic of NTPases and 2 potential transmembrane segments at the N- and C-terminal ends. Northern blot analysis revealed that CD39L3 is expressed as an approximately 3-kb mRNA in most tissues. A weaker signal of approximately 1.8 kb was detected in pancreas. Homology searches yielded ESTs that likely represented the mouse cd39l3 gene.
By assaying cellular extracts of transfected COS-7 cells, Munkonda et al. (2007) showed that all P2 receptor antagonists tested, except for MRS2179, an AMP analog, inhibited human and mouse plasma membrane-bound ENTPDases, including ENTPD3. ENTPD3 was most sensitive to inhibition among the ENTPDases tested.
By analysis of radiation hybrid and somatic cell hybrid panels, Chadwick and Frischauf (1998) mapped the CD39L3 gene to 3p21.3.
Chadwick, B. P., Frischauf, A.-M. The CD39-like gene family: identification of three new human members (CD39L2, CD39L3, and CD39L4), their murine homologues, and a member of the gene family from Drosophila melanogaster. Genomics 50: 357-367, 1998. [PubMed: 9676430] [Full Text: https://doi.org/10.1006/geno.1998.5317]
Munkonda, M. N., Kauffenstein, G., Kukulski, F., Levesque, S. A., Legendre, C., Pelletier, J., Lavoie, E. G., Lecka, J., Sevigny, J. Inhibition of human and mouse plasma membrane bound NTPDases by P2 receptor antagonists. Biochem. Pharm. 74: 1524-1534, 2007. [PubMed: 17727821] [Full Text: https://doi.org/10.1016/j.bcp.2007.07.033]