Gene: [06p12/VEGF] vascular endothelial growth factor;

SUM	[1] Function: growth factor active in angiogenesis, particularily in endothelial cell growth. Subunit stucture: homodimer, disulfide-linked. Transcription: includes alternative splicing causing a formation of four distinctive polypeptide forms of VEGF - 121, 165, 189, and 206 amino acids. Sequence similarity: to platelet-derived growth factor alpha and beta polypeptides (GEM:07p22/PDGFA; GEM:22q1/PDGFB). [2] Recent History: - According to D.W.Leung et al., 1989 (Dept Mol Biol, Genetech, South San Francisco, CA 94080), vascular endothelial growth factor (VEGF) is a hep- arin-binding growth factor specific for vascular endothelial cells that is able to induce angiogenesis in vivo. Complementary DNA clones for bovine and human VEGF were isolated from cDNA libraries prepared from FC and HL60 leukemia cells, respectively. These cDNAs encode hydrophilic proteins with sequences related to those of the A and B chains of platelet-derived growth factor. DNA sequencing suggests the existence of several molecular species of VEGF. VEGFs are secreted proteins, in contrast to other endothelial cell mitogens such as acidic or basic FGFs and platelet-derived endothelial cell growth factor. - According to E.Tischer et al., 1991 (California Biotech. Inc., Mountain View 94043), VEGF is an endothelial cell-specific mitogen, in particularly produced by cultured vascular smooth muscle cells. Analysis of VEGF tran- scripts in these cells revealed three different forms of VEGF cDNAs encod- ing three distinctive forms of the human VEGF polypeptides - 189, 165, and 121 amino acids in length. Comparison of the cDNA sequences with sequences derived from human VEGF genomic clones indicates that the VEGF gene con- tains eight exons and that the various VEGF transcripts arise by ALTERNA- TIVE SPLICING: in particularly, the 165-aa form is missing the residues encoded by exon 6, and the 121-aa form is missing the residues encoded by exons 6 and 7. The VEGF gene promoter region contains a single major tran- scription start site lying near a cluster of potential Sp1 factor binding sites. The promoter region also contains several potential binding sites for the transcription factors AP-1 and AP-2. - According to K.A.Houck et al., 1991 (Dept Mol Biol, Genetech, Inc., South San Francisco, California 94080), VEGF is a secreted, direct-acting mitogen specific for vascular endothelial cells and capable of stimulating angiogen- esis in vivo. Molecular cloning revealed multiple forms of VEGF, apparently arising from ALTERNATIVE SPLICING of its RNA transcript. The authors have examined various human cDNA libraries by the polymerase chain reaction tech- nique and discovered a FOURTH MOLECULAR FORM, VEGF-206. This form contains a 41-amino acid insertion relative to the most abundant form, VEGF-165, and includes the highly basic 24-amino acid insertion found in VEGF-189. South- ern blot analysis revealed that a single gene encoded these various forms, and nucleic acid sequence analysis of a portion of the VEGF gene revealed an intron/exon structure compatible with alternative splicing of RNA as a mechanism for their generation. Transient transfection of human embryonic kidney 293 cells showed that, like VEGF-189, VEGF-206 was predominately cell-associated and only very poorly secreted despite the presence of the SIGNAL PEPTIDE IDENTICAL TO THAT FOUND IN VEGF-121 AND VEGF-165, both of which are efficiently exported from the cell. Vascular permeability activ- ity was detected in the medium of 293 cells transfected with all four forms of VEGF; however, endothelial cell mitogenic activity was apparent only with VEGF-121 and VEGF-165. The authors concluded that alternative splicing of VEGF RNA can produce four polypeptides with strikingly differ- ent secretion patterns, which suggests multiple physiological roles for this family of proteins."
PRT	"VEGF precursor protein: =215 aa; 25173 Da. Sources: - Leung DW, Cachianes G, Kuang W-J, Goeddel DV, Ferrara N; Science 246, 1306-1309, 1989. - Keck PJ, Hauser SD, Krivi G, Sanzo K, Warren T, Feder J, Connolly DT; Science, 246, N? (Dec 8), 1309-1312, 1989. - Connolly DT, Olander JV, Heuvelman D, Nelson R, Monsell R, Siegel N, Haymore BL, Leimgruber R, Feder J; JBC, 264, 20017-20024, 1989; /Note: AA-Seq of 27-36, 43-50, and 59-81 aa residues/. - Tischer E, Mitchell R, Hartman T, Silva M, Gospodarowicz D, Fiddes JC, Abraham JA; JBC, 266, N18 (Jun 25), 11947-11954, 1991. - Houck KA, Ferrara N, Winer J, Cachianes G, Li B, Leung DW; Mol Endocrinol, 5, N12, 1806-1814, 1991; /Note: AA-Seq of VEGF-206 does not include the 26 aa signal segment and the first 86 aa residues of its mature form/. - Weindel K, Marme D, Weich HA; BBRC, 183, 1167-1174, 1992. - Fiebich BL, Jaeger B, Schoellmann C, Weindel K, Wilting J, Kochs G, Marme D, Hug H, Weich HA; Eur J Biochem, 211, 19-26, 1993; /Note: AA-Seq of 27-41 aa residues/. ...................................................................... PRT:{-26( 1)=MNFLLSWVHW SLALLLYLHH AKWSQA= 1( 27)=APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS= 51( 77)=CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN= 101(127)=KCECRPKKDR ARQEK= 116(142)=KSVRGKGKGQ KRKRKKSRYK SWSV= /missing in VEGF-165/121 140(166)=YVGARCCLMP WSLPGPH= /missing in VEGF-189/165/121 157(183)=PCGPCSERRK HLFVQDPQTC KCSCKNT= /missing in VEGF-121 184(210)=DSRCKARQLE LNERTCR= /missing in VEGF-121 201(227)=CDKPRR=206/189/165/121(232)} ...................................................................... Features: {seg(-26..-1) = SIGNAL-peptide; seg(1..206) = MATURE chain; pos(75=N/Asn) = CARBOHYD (potential); pos(115) = substitution: K -> N in VEGF-121 and VEGF-165; seg(116..200) = alt_splised: missing in VEGF-121; seg(116..156) = alt_spliced: missing in VEGF-165; seg(140..156) = alt_spliced: missing in VEGF-189}"
COD	"[1] Sources: - (1) cDNA =649 bp; Weindel K, Folkman J, Morme D, Weich H; AIDS-associated Kaposi's sarcoma cells in culture express VEGF /Note: cell_type: KS-3 (AIDS-KS-3); Submitted (Oct-10-1991) to EMBL/GenBank by Weich HA (Univ. of Freiburg, Mol. Cell Biol., c/o Goedecke AG, Mooswaldallee 1-9, 7800 Freiburg, FRG); DB_Entry (EMBL): emb:X62568/HSVEGF/Oct-16-1991, also referring to Weindel K, Marme D, Weich HA; BBRC, 183, 1167-1174, 1992. - (2) cDNA =990 bp; Leung DW, Cachianes G, Kuang W-J, Goeddel DV, Ferrara N; Vascular endothelial growth factor is a secreted angiogenic mitogen; Science, 246, N? (Dec 8), 1306-1309, 1989; DB_Entry (GenBank): gnb:M32977/HUMEGFAA/Feb-28-1991; Human heparin-binding vascular endothelial growth factor (VEGF) mRNA, complete cds (angiogenic mitogen) /Note: Human promyelocytic leukemia cell line HL60. - (3) cDNA =1195 bp; Keck PJ, Hauser SD, Krivi GG, Sanzo K, Warren T, Feder J, Connolly DT; Vascular permeability factor, an endothelial cell mitogen related to PDGF; Science 246, N? (Dec 8), 1309-1312, 1989; Submitted (Aug-22-1989) to EMBL/GenBank by Keck PJ; DB_Entry (GenBank): gnb:M27281=X15997/HUMVPF/Jun-15-1990; Human vascular permeability factor mRNA, complete cds (endothelial cell mitogen) /Note: histiocytic lymphoma cell line U937; clone hVPF4. - (4) gDNA from Tischer-1991 (see the topic 'Gene_structure'). - (5) gDNA from Houck KA, Ferrara N, Winer J, Cachianes G, Li B, Leung DW; The vascular endothelial growth factor family: identification of a fourth molecular species and characterization of alternative splicing of RNA Mol Endocrinol, 5, N12, 1806-1814 (1991) %---------- [2] Features: 'start' 'stop' (4) gDNA/contig_1&8:CDS(1:3401=ATGaa.. ..gg_8:37=TGA)gc.. (Tischer-1991) (3) M27281/1195 bp :CDS( 157=ATGaa.. ..gg__802=TGA)gc.. (Keck-1989) (2) M32977/ 990 bp :CDS( 57=ATGaa.. ..gg__630=TGA)gc.. (Leung-1989) (1) X62568/ 649 bp :CDS( 17=ATGaa.. ..gg__590=TGA)gc.. (Weindel-1992) %---------- gDNA(1):3237=gcgc.. 3251=ccagccgcgc.. 3271=ggccctggcc cgggcctcgg= cDNA(3): 1=gcgc.. 15=ccagc-gcgc.. 34=g-ccctg-cc cgg-cctcgg= %---------- gDNA(1):3291=gccggggagg.. ..3341=cacagcccga gccggagagg= cDNA(3): 51=gccggg-agg.. .. 100=cacagcccga gccggagagg= cDNA(2): .___________________1=__cagTGTgCTgGcggCCCgg= %---------- gDNA(1):3361=gagcgcgagc cgcgccggcc ccggtcgggc.. 3401=atg.. 3434=cttg= cDNA(3): 120=ga-cgcgagc cgcgc-g-cc ccggtcgggc.. _157=atg.. _190=cttg= cDNA(2): 20=___cgcgagc cgGCccggcc ccggtcgggc.. __57=atg.. __90=ctCg= cDNA(1): 1=__________________________tcgggc.. __17=atg.. __50=cttg= %---------- exon_6: alternatively spliced segment missing in the cDNAs (2)&(1): %---------- gDNA(6): 18=aaaatcagtt cgaggaaagg gaaaggggca aaaacgaaag= cDNA(3):579=aaaatcagtt cgaggaaagg gaaaggggca aaaacgaaag= %---------- gDNA(6): 58=cgcaagaaat cccggtataa gtcctggagc gt=89/end cDNA(3):619=cgcaagaaat cccggtataa gtcctggagc gt=650/end %---------- 3'-untranslated segments: %---------- gDNA(8): 68=gggtttcgg=76/END cDNA(3):833=gggtttcggg .. 853=ctcaccagga aagactgata=.. cDNA(2):661=gggtttcggg .. 681=ctcaccagga aagactgata=.. cDNA(1):621=gCgtttcggg .. 641=ctcaccagg=649/END %---------- cDNA(3):1133=tgtagacaca cc_cacccaca tacatacatt= cDNA(2): 961=tgtagacaca cc_gcggccgc cagcacactg=990/END %---------- cDNA(3):1163=tatatatata tatattatat atatataaat taa=1195/END"
GEN	[1] gcDNA = 4674 bp. Sources: (1) Tischer E, Mitchell R, Hartman T, Silva M, Gospodarowicz D, Fiddes JC, Abraham JA; The human gene for vascular endothelial growth factor: Multiple protein forms are encoded through alternative exon splicing; J Biol Chem, 266, N18 (Jun 25), 11947-11954, 1991. - DB_Entries (GenBank/EMBL): {gnb:M63971..M63978/HUMVEGF1..F8/Jul-19-1991; Human vascular endothelial growth factor gene /Note: cell =fibroblast; tissue =lung} (2) Keck PJ, Hauser SD, Krivi GG, Sanzo K, Warren T, Feder J, Connolly DT; Vascular permeability factor, an endothelial cell mitogen related to PDGF; Science 246, N? (Dec 8), 1309-1312, 1989. - DB_Entry (GenBank/EMBL): {gnb:M27281=X15997/HUMVPF/Jun-15-1990; Human vascular permeability factor mRNA, com- plete cds (endothelial cell mitogen) /Note: histiocytic lymphoma cell line U937; clone hVPF4}. %---------- [2] Structure: gene(=?) /Note: known regions encompass 4674 bp/ Features: 5'-NTC(=2362): {contig_1(1..2362); MPR(=242?): {contig_1(2121?..2362?); /Note: this 'Major Promoter Region' lacks any TATA-like boxes but contains one perfect SP1 element , one incom- plete SP1 element , and several repeats that are complementary to a sequence of the right half of SP1} TCI_1(=1): {contig_1(2363=t) /Note: cap-site?} /Note-1: the 1195 bp-cDNA/mRNA species (gnb:M27281/Keck-1989) starts within exon_1 at the position contig_1(3237=gc); conceptual translation of this mRNA starts 157 bp downstream at the position contig_1(3401=atg); Note-2: the 990 bp-cDNA/mRNA species (gnb:M32977/Leung-1989) starts within exon_1 at the position contig_1:(3343=ca); conceptual translation of this mRNA starts 57 bp downstream at the position contig_1(3401=atg); Note-3: the 649 bp-cDNA/mRNA species (emb:X62568/Weindel-1992) starts within exon_1 at the position contig_1(3385=tc); con- ceptual translations of this mRNA starts 17 bp downstream at the position contig_1(3401=atg); Note-4: also see, in the section 'EnCoding_region', comments to a multiple alignment of all cDNAs involved, which reveals several deletions, insertions, and single nucleotide substi- tutions presented in particular cDNA species/ exon_1(=1104): {contig_1(2363..3466); 5'-UTL(=1038): contig_1(2363..3400); TLI_1(=3): contig_1(3401=atg=3403); cds_1(=66): contig_1(3401..3466); pep_1(=23): prp(1..23)} exon_2(=52): {contig_2(18..69); pep_2(=17.3):prp(24..40.3); PRT_1,2,3(1..14.3)} exon_3(=197): {contig_3(18..214); pep_3(=66): prp(41..106); PRT_1,2,3(15..80)} exon_4(=77): {contig_4(18..94); pep_4(=25.7):prp(107..131.7); PRT_1,2,3(81..105.7)} exon_5(=30): {contig_5(18..47); pep_5(=10.7):prp(132..141.7); PRT_1,2,3(106..115.7)} exon_6(=72): {contig_6(18..89); pep_6(=24.7):prp(142..165.7); PRT_1(116..139.7)} exon_7(=132): {contig_7(18..149); pep_7(=44.7):prp(166..209.7); PRT_1(140..183.7); PRT_2(116..159.7)} exon_8(=413): {contig_8(18..430); cds_8(=19): contig_8(18..36); pep_8(=7): prp(210..216); PRT_1(184..190); PRT_2(160..166); PRT_3(116..122); TLT_1(=3): contig_8(37=tga=39); 3'-UTL(=394): contig_8(37..430); TTS_1(=5)?: contig_8(at_422=ATAAA=426_tt) /Note: this segment can only be considered a polyA-signal- -LIKE sequence (i.e., a suggestive Transcription Termination Signal, TTS/; TCT_1(=1)?: contig_8(430=a) /Note: putative} intron_1^2(=?): {intron_1^2.up(=14):contig_1(3467..3480); gap_1,2(=?); intron_1^2.dn(=17):contig_2(1..17)} intron_2^3(=?): {intron_2^3.up(=17):contig_2(70..86); gap_2,3(=?); intron_2^3.dn(=17):contig_3(1..17)} intron_3^4(=?): {intron_3^4.up(=17):contig_3(215..231); gap_3,4(=?); intron_3^4.dn(=17):contig_4(1..17)} intron_4^5(=?): {intron_4^5.up(=17):contig_4(95..111); gap_4,5(=?); intron_4^5.dn(=17):contig_5(1..17)} intron_5^6(=?): {intron_5^6.up(=17):contig_5(48..64); gap_5,6(=?); intron_5^6.dn(=17):contig_6(1..17)} intron_6^7(=?): {intron_6^7.up(=17):contig_6(90..106); gap_6,7(=?); intron_6^7.dn(=17):contig_7(1..17)} intron_7^8(=?): {intron_7^8.up(=17):contig_7(150..166); gap_7,8(=?); intron_7^8.dn(=17):contig_8(1..25)} 3'-NTC(=?): {?} %---------- [3] Sequence: {contig_1: 1=gaattctgtg ccctcactcc cctggatccc tgggcaaagc cccagaggga aacacaaaca= 61=ggttgttgta acacaccttg ctgggtacca ccatggagga cagttggctt atgggggtgg= 121=ggggtgcctg gggccacgga gtgactggtg atggctatcc ctccttggaa cccctccagc= 181=ctcctcttag cttcagattt gtttatttgt tttttactaa gacctgctct ttcaggtctg= 241=ttggctcttt taggggctga agaaggccga gttgagaagg gatgcaaggg agggggccag= 301=aatgagccct tagggctcag agcctccatc ctgccccaag atgtctacag cttgtgctcc= 361=tggggtgcta gaggcgcaca aggaggaaag ttagtggctt cccttccata tcccgttcat= 421=cagcctagag catggagccc aggtgaggag gcctgcctgg gagggggccc tgagccagga= 481=aataaacatt tactaactgt acaaagacct tgtccctgct gctggggagc ctgccaagtg= 541=gtggagacag gactagtgca cgaatgatgg aaagggaggg ttggggtggg tgggagccag= 601=cccttttcct cataagggcc ttaggacacc ataccgatgg aactgggggt actggggagg= 661=taacctagca cctccaccaa accacagcaa catgtgctga ggatggggct gactaggtaa= 721=gctccctgga gcgttttggt taaattgagg gaaattgctg cattcccatt ctcagtccat= 781=gcctccacag aggctatgcc agctgtaggc cagaccctgg cacgatctgg gtggataatc= 841=agactgactg gcctcagagc cccaactttg ttccctgggg cagcctggaa atagccaggt= 901=cagaaaccag ccaggaattt ttccaagctg cttcctatat gcaagaatgg gatgggggcc= 961=tttgggagca cttagggaag atgtggagag ttggaggaaa agggggcttg gaggtaaggg= 1021=aggggactgg gggaaggata ggggagaagc tgtgagcctg gagaagtagc caagggatcc= 1081=tgagggaatg ggggagctga gacgaaaccc ccatttctat tcagaagatg agctatgagt= 1141=ctgggcttgg gctgatagaa gccttggccc ctggcctggt gggagctctg ggcagctggc= 1201=ctacagacgt tccttagtgc tggcgggtag gtttgaatca tcacgcaggc cctggcctcc= 1261=acccgccccc accagccccc tggcctcagt tccctggcaa catctggggt tgggggggca= 1321=gcaggaacaa gggcctctgt ctgcccagct gcctccccct ttgggttttg ccagactcca= 1381=cagtgcatac gtgggctcca acaggtcctc ttccctccca gtcactgact aaccccggaa= 1441=ccacacagct tcccgttctc agctccacaa acttggtgcc aaattcttct cccctgggaa= 1501=gcatccctgg acacttccca aaggacccca gtcactccag cctgttggct gccgctcact= 1561=ttgatgtctg caggccagat gagggctcca gatggcacat tgtcagaggg acacactgtg= 1621=gcccctgtgc ccagccctgg gctctctgta catgaagcaa ctccagtccc aaatatgtag= 1681=ctgtttggga ggtcagaaat agggggtcca ggagcaaact ccccccaccc cctttccaaa= 1741=gcccattccc tctttagcca gagccggggt gtgcagacgg cagtcactag ggggcgctcg= 1801=gccaccacag ggaagctggg tgaatggagc gagcagcgtc ttcgagagtg aggacgtgtg= 1861=tgtctgtgtg ggtgagtgag tgtgtgcgtg tggggttgag ggtgttggag cggggagaag= 1921=gccaggggtc actccaggat tccaatagat ctgtgtgtcc ctctccccac ccgtccctgt= 1981=ccggctctcc gccttcccct gcccccttca atattcctag caaagaggga acggctctca= 2041=ggccctgtcc gcacgtaacc tcactttcct gctccctcct cgccaatgcc ccgcgggcgc= 2101 gtgtctctgg acagagtttc cgggggcgga tgggtaattt tcaggctgtg aaccttggtg= 2161=ggggtcgagc ttccccttca ttgcggcggg ctgcgggcca ggcttcactg ggcgtccgca= 2221=gagcccgggc ccgagccgcg tgtggagggg ctgaggctcg cctgtccccg ccccccgggg= 2281=cgggccgggg gcggggtccc ggcggggcgg agccatgcgc cccccccttt tttttttaaa= 2341=agtcggctgg tagcggggag ga= 2363=Tcgcggaggc ttggggcagc cgggtagctc ggaggtcgtg gcgctggggg ctagcaccag= 2423=cgctctgtcg ggaggcgcag cggttaggtg gaccggtcag cggactcacc ggccagggcg= 2483=ctcggtgctg gaatttgata ttcattgatc cgggttttat ccctcttctt ttttcttaaa= 2543=catttttttt taaaactgta ttgtttctcg ttttaattta tttttgcttg ccattcccca= 2603=cttgaatcgg gccgacggct tggggagatt gctctacttc cccaaatcac tgtggatttt= 2663=ggaaaccagc agaaagagga aagaggtagc aagagctcca gagagaagtc gaggaagaga= 2723=gagacggggt cagagagagc gcgcgggcgt gcgagcagcg aaagcgacag gggcaaagtg= 2783=agtgacctgc ttttgggggt gaccgccgga gcgcggcgtg agccctcccc cttgggatcc= 2843=cgcagctgac cagtcgcgct gacggacaga cagacagaca ccgcccccag ccccagctac= 2903=cacctcctcc ccggccggcg gcggacagtg gacgcggcgg cgagccgcgg gcaggggccg= 2963=gagcccgcgc ccggaggcgg ggtggagggg gtcggggctc gcggcgtcgc actgaaactt= 3023=ttcgtccaac ttctgggctg ttctcgcttc ggaggagccg tggtccgcgc gggggaagcc= 3083=gagccgagcg gagccgcgag aagtgctagc tcgggccggg aggagccgca gccggaggag= 3143=ggggaggagg aagaagagaa ggaagaggag agggggccgc agtggcgact cggcgctcgg= 3203=aagccgggct catggacggg tgaggcggcg gtgtgcgcag acagtgctcc agccgcgcgc= 3263=gctccccagg ccctggcccg ggcctcgggc cggggaggaa gagtagctcg ccgaggcgcc= 3323=gaggagagcg ggccgcccca cagcccgagc cggagaggga gcgcgagccg cgccggcccc= 3383=ggtcgggcct ccgaaacc= 3401=ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA= 3451=CCTCCACCAT GCCAAG= 3467=gtaagcggtc gtgc=3480} {contig_2:1=tctctttctg tcctcag= 18=TGGTCCCAGG CTGCACCCAT GGCAGAAGGA GGAGGGCAGA ATCATCACGA= 68=AG= 70=gtgagtcccc ctggctg=86} {contig_3:1=catcgcctct catgcag= 18=TGGTGAAGTT CATGGATGTC TATCAGCGCA GCTACTGCCA TCCAATCGAG= 68=ACCCTGGTGG ACATCTTCCA GGAGTACCCT GATGAGATCG AGTACATCTT= 118=CAAGCCATCC TGTGTGCCCC TGATGCGATG CGGGGGCTGC TGCAATGACG= 168=AGGGCCTGGA GTGTGTGCCC ACTGAGGAGT CCAACATCAC CATGCAG= 215=gtgggcatct ttgggaa=231} {contig_4:1=gcttccttcc tttccag= 18=ATTATGCGGA TCAAACCTCA CCAAGGCCAG CACATAGGAG AGATGAGCTT= 68=CCTACAGCAC AACAAATGTG AATGCAG= 95=gtgaggatgt agtcacg=111} {contig_5:1=ctccctaccc attgcag= 18=ACCAAAGAAA GATAGAGCAA GACAAGAAAA= 48=gtaagtggcc ctgactt=64} {contig_6:1=gtttttttat tttccag= 18=AAAATCAGTT CGAGGAAAGG GAAAGGGGCA AAAACGAAAG CGCAAGAAAT= 68=CCCGGTATAA GTCCTGGAGC GT= 90=gtacgttggt gcccgct=106} {contig_7:1=cttttgcctt tttgcag= 18=TCCCTGTGGG CCTTGCTCAG AGCGGAGAAA GCATTTGTTT GTACAAGATC= 68=CGCAGACGTG TAAATGTTCC TGCAAAAACA CAGACTCGCG TTGCAAGGCG= 118=AGGCAGCTTG AGTTAAACGA ACGTACTTGC AG= 150=gttggttccc agaggca=166} {contig_8:1=ttttccattt ccctcag= 18=ATGTGACAAG CCGAGGCGG= *37=TGAgccgggc aggaggaagg agcctccctc agggtttcgg=76 77=gaaccagatc tctcaccagg aaagactgat acagaacgat cgatacagaa accacgctgc= 137=cgccaccaca ccatcaccat cgacagaaca gtccttaatc cagaaacctg aaatgaagga= 197=agaggagact ctgcgcagag cactttgggt ccggagggcg agactccggc ggaagcattc= 257=ccgggcgggt gacccagcac ggtccctctt ggaattggat tcgccatttt atttttcttg= 317=ctgctaaatc accgagcccg gaagattaga gagttttatt tctgggattc ctgtagacac= 377=acccacccac atacatacat ttatatatat atatattata tatatataaa ttaa=430} %---------- [4] Constraints: {contig =8; gap_i,j =7; intron_i^j.up =7; intron_i^j.dn =7; \Rule-1: intron_i^j ==(intron_i^j.up & gap_i,j & intron_i^j.dn)\ 5'-NTC =1; uSPE =?; MPR =1; TCI =1>?; mRNA =4; 5'-UTL =1>?; 3'-UTL =1; exon =8; TLI =1; TLT =1; \Rule-2: cds_1
ECR	"[1] Cross-References to Protein DBs: swp:P15692/VEGF_HUMAN/Apr-01-1990/Jul-01-1993. pir:A34492; pir:A40079; pir:A40080; pir:A40454; pir:B40454; pir:C40454; pir:JQ1463; pir:JQ1464; pir:S17348; prosite:PS00249/PDGF. [2] Cross-References to DNA DBs: {emb:M27281=X15997/HSVPF; emb:M32977/HSEGFAA; emb:X62568/HSVEGF. emb:M63971/HSVEGF1; emb:M63972/HSVEGF2; emb:M63973/HSVEGF3; emb:M63974/HSVEGF4; emb:M63975/HSVEGF5; emb:M63976/HSVEGF6; emb:M63977/HSVEGF7; gnb:M63978/HUMVEGF8; gnb:S85199/S85199S1; gnb:S85201/S85199S2; gnb:S85219/S85199S3; gnb:S85222/S85199S4; gnb:S85224/S85199S5}"
FAG	The VEGF receptor is mapped to Chr 4q12 (GEM:04q12/KDR).
REL	GEM:11q13/VEGFB; GEM:00.0/VEGFC; GEM:0Xp2231/FIGF.
REF	FUN,EXP,MEB "Berse B &: Mol Cell Biol, 3, N2 (Feb), 211-220, 1992 MOD,MOU,PAT "Carmellet P &: Nature, 380, 435-439, 1996 CLO,SEQ,COD,PEP "Connolly DT &: JBC, 264, 20017-20024, 1989 MOD,MOU,PAT "Ferrara N &: Nature, 380, 439-442, 1996 FUN,EXP,MEB "Ferrara N &: Endocrine Rev, 13, N1 (Feb), 18-32, 1992 FUN,EXP,MEB "Ferrara N &: Growth Factors, 5, N2, 141-148, 1991a FUN,EXP,MEB "Ferrara N &: J Cell Biochem, 47, N3 (Nov), 211-218, 1991b CLO,SEQ,COD,PEP "Fiebich BL &: Eur J Biochem, 211, 19-26, 1993a FUN,MOP,STR "Fiebich BL &: Eur J Biochem, 211, N1-2 (Jan 15), 19-26, 1993b CLO,GEN,STR "Houck KA &: Mol Endocrinol, 5, N12, 1806-1814, 1991 CLO,SEQ,COD,PEP "Keck PJ &: Science, 246, (Dec 8), 1309-1312, 1989 FUN,MOP,STR "Kim KJ &: Growth Factors, 7, N1, 53-64, 1992 CLO,SEQ,COD,PEP "Leung DW &: Science, 246, (Dec 8), 1306-1309, 1989 LOC "Mattei M-G &: Genomics, 32, 168-169, 1996 FUN,EXP,MEB "Park JE &: Mol Cell Biol, 4, N12, 1317-1326, 1993 FUN,EXP,MEB "Ravindranath N &: Endocrinology, 131, N1 (Jul), 254-260, 1992 FUN,EXP,MEB "Sharkey AM &: J Reprod Fert, 99, N2 (Nov), 609-615, 1993 CLO,GEN,STR "Tischer E &: JBC, 266, N18 (Jun 25), 11947-11954, 1991 LOC "Wei M-H &: Hum Genet, 97, 794-797, 1996 CLO,SEQ,COD,PEP "Weindel K &: BBRC, 183, 1167-1174, 1992 FUN,EXP,MEB "Yan Z &: J Clin Endocrinol Metab, 77, N6, 1723-1725, 1993 FUN,MOP,STR "Yeo TK &: BBRC, 179, N3 (Sep 30), 1568-1575, 1991
SWI	SWISSPROT: P15692
KEY	cyk, hem, card
CLA	coding, basic
LOC	06 p12
MIM	MIM: 192240

Смотрите также:

Фактор роста сосудистого эндотелия (VEGF)

Ангиогенез опухлей: роль p53

Ангиогенез опухлей: роль экспрессии VHL